Patents by Inventor David Sukovich

David Sukovich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multiplex capture of gene and protein expression from a biological sample
    Patent number: 11970739
    Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.
    Type: Grant
    Filed: July 6, 2023
    Date of Patent: April 30, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
  • Methods, compositions, and systems for capturing probes and/or barcodes
    Patent number: 11959076
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Grant
    Filed: August 11, 2023
    Date of Patent: April 16, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori
  • Methods, compositions, and systems for spatial analysis of analytes in a biological sample
    Patent number: 11873482
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Grant
    Filed: April 27, 2023
    Date of Patent: January 16, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori, Lauren Gutgesell, Janine Hensel, Seayar Mohabbat
  • MULTIPLEX CAPTURE OF GENE AND PROTEIN EXPRESSION FROM A BIOLOGICAL SAMPLE
    Publication number: 20240011090
    Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.
    Type: Application
    Filed: July 6, 2023
    Publication date: January 11, 2024
    Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR IMPROVED IN SITU DETECTION OF ANALYTES AND SPATIAL ANALYSIS
    Publication number: 20230416808
    Abstract: Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.
    Type: Application
    Filed: September 7, 2023
    Publication date: December 28, 2023
    Inventors: David Sukovich, Felice Alessio Bava, Augusto Manuel Tentori, Hanyoup Kim, Amanda Janesick
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR CAPTURING PROBES AND/OR BARCODES
    Publication number: 20230383285
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Application
    Filed: August 11, 2023
    Publication date: November 30, 2023
    Applicant: 10x Genomics, Inc.
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori
  • METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
    Publication number: 20230340565
    Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.
    Type: Application
    Filed: January 17, 2023
    Publication date: October 26, 2023
    Inventors: Zahra Kamila BELHOCINE, Josephine Lee, Francesca MESCHI, Luz MONTESCLAROS, Katherine PFEIFFER, Andrew D. PRICE, Jerald SAPIDA, David SUKOVICH, Tobias Daniel WHEELER, Yifeng YIN
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR SPATIAL ANALYSIS OF ANALYTES IN A BIOLOGICAL SAMPLE
    Publication number: 20230332138
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Application
    Filed: April 27, 2023
    Publication date: October 19, 2023
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori, Lauren Gutgesell, Janine Hensel, Seayar Mohabbat
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR CAPTURING ANALYTES FROM GLIOBLASTOMA SAMPLES
    Publication number: 20230332247
    Abstract: Provided herein are methods, compositions and systems for identifying spatial gene expression of analytes from glioblastoma derived tissue. The methods discloses herein include using templated ligation probe pairs to identify location of a disease proliferating region in a glioblastoma-derived sample by detecting analytes in the region and hybridizing a ligation product comprising the probe pairs to a capture probe on a spatial array.
    Type: Application
    Filed: June 23, 2023
    Publication date: October 19, 2023
    Inventors: Hardeep Pal Singh, David Sukovich, Seayar Mohabbat, Dongyao Li, Hanyoup Kim, Augusto Manuel Tentori
  • METHODS, COMPOSITIONS, KITS, AND SYSTEMS FOR ENHANCING ANALYTE CAPTURE FOR SPATIAL ANALYSIS
    Publication number: 20230295699
    Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.
    Type: Application
    Filed: May 3, 2023
    Publication date: September 21, 2023
    Inventors: David Sukovich, Hanyoup Kim, Jerald Sapida, Spontaneous Rose McKnight Russell, Layla Katiraee, Augusto Manuel Tentori
  • Multiplex capture of gene and protein expression from a biological sample
    Patent number: 11739381
    Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.
    Type: Grant
    Filed: July 18, 2022
    Date of Patent: August 29, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR CAPTURING PROBES AND/OR BARCODES
    Publication number: 20230203478
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Application
    Filed: March 3, 2023
    Publication date: June 29, 2023
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori
  • Methods, compositions, and systems for spatial analysis of analytes in a biological sample
    Patent number: 11680260
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Grant
    Filed: October 28, 2022
    Date of Patent: June 20, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori, Lauren Gutgesell, Janine Hensel, Seayar Mohabbat
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR SPATIAL ANALYSIS OF ANALYTES IN A BIOLOGICAL SAMPLE
    Publication number: 20230113230
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Application
    Filed: October 28, 2022
    Publication date: April 13, 2023
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori, Lauren Gutgesell, Janine Hensel, Seayar Mohabbat
  • Methods, compositions, and systems for capturing probes and/or barcodes
    Patent number: 11618897
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Grant
    Filed: July 22, 2022
    Date of Patent: April 4, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori
  • Methods and systems for sample preparation and analysis
    Patent number: 11584954
    Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.
    Type: Grant
    Filed: April 22, 2020
    Date of Patent: February 21, 2023
    Assignee: 10X GENOMICS, INC.
    Inventors: Zahra Kamila Belhocine, Josephine Lee, Francesca Meschi, Luz Montesclaros, Katherine Pfeiffer, Andrew D. Price, Jerald Sapida, David Sukovich, Tobias Daniel Wheeler, Yifeng Yin
  • MULTIPLEX CAPTURE OF GENE AND PROTEIN EXPRESSION FROM A BIOLOGICAL SAMPLE
    Publication number: 20220389504
    Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.
    Type: Application
    Filed: July 18, 2022
    Publication date: December 8, 2022
    Inventors: Jennifer Chew, Joseph Francis Shuga, Patrick Roelli, Denise Cheung, Alexander Hermes, Yifeng Yin, Naishitha Anaparthy, Ryo Hatori, Marlon Stoeckius, Rapolas Spalinskas, Anna-Maria Katsori, Cedric Uytingco, Mesruh Turkekul, David Sukovich, Christina Galonska, Aleksandra Jurek, Octavian Marian Bloju
  • METHODS, COMPOSITIONS, AND SYSTEMS FOR CAPTURING PROBES AND/OR BARCODES
    Publication number: 20220356464
    Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.
    Type: Application
    Filed: July 22, 2022
    Publication date: November 10, 2022
    Inventors: Hanyoup Kim, David Sukovich, Layla Katiraee, Augusto Manuel Tentori
  • COMBINED MULTIPLE-DISPLACEMENT AMPLIFICATION AND PCR IN AN EMULSION MICRODROPLET
    Publication number: 20220154248
    Abstract: The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.
    Type: Application
    Filed: September 9, 2021
    Publication date: May 19, 2022
    Inventors: Adam R. Abate, David Sukovich
  • Combined multiple-displacement amplification and PCR in an emulsion microdroplet
    Patent number: 11142791
    Abstract: The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.
    Type: Grant
    Filed: August 9, 2017
    Date of Patent: October 12, 2021
    Assignee: The Regents of the University of California
    Inventors: Adam R. Abate, David Sukovich