Abstract: A method for overexpressing a bioelastic polypeptide in a host cell is provided. A nucleic acid encoding a bioelastic polypeptide having pentapeptide, tetrapeptide, hexapeptide or nonapeptide repeating units is introduced into a host cell. The host cell is grown under conditions that provide for expression of the polypeptide as at least 40% of the total cellular protein of the host cell.

Abstract: A method for purifying an artificial polymer that exhibits a reversible inverse temperature transition is provided. The method involves (a) dissolving the polymer in an aqueous medium so that the temperature of the medium is below the effective transition temperature; (b) adjusting the temperature of the aqueous medium relative to the effective transition temperature of the polymer; (c) removing any particulate material from the medium; (d) adjusting the temperature of the aqueous medium relative to the effective transition temperature of the polymer so that the temperature of the medium is above the effective transition temperature; (e) collecting the polymer from the medium as a more dense phase; and (f) optionally repeating any of steps (a)-(e) until a desired level or purity is reached; with the proviso the order of steps can be (a)-(d)-(e)-(a)-(b)-(c).

Abstract: A mailing system is includes of a mailing machine operating under the control of a microcontroller having a communication port with a first channel and a second channel. The mailing system also includes a meter vault which operates under the control a microcontroller mounted in a secure housing and having a communication port with a first channel and a second channel. A printer is included which operates under the control of a microcontroller mounted in a secure housing and having a communication port having a first channel and a second channel. A printer interface is included for providing a number of independent communication paths. A first path provides communication between first channel of the mailing machine and the first channel of the meter vault. A second path provides communication between the second channel of the mailing machine and the second channel of the printer, and a third path for providing communications between the second channel of meter vault and the first channel of the printer.