Abstract: Multiplex PCR-based assays may be improved by using a load-balanced set of primers with different proportions of primers in the set to provide balanced amplicon read depth. The multiplex PCR amplification in such assays may be improved by using primers with exonuclease-resistant bonds or nucleotides to reduce the formation of primer dimers.

Abstract: Multiplex PCR amplification may be improved by using primers with exonuclease-resistant bonds or nucleotides to reduce the formation of primer dimers. Multiplex PCR-based assays may be improved by using a load-balanced set of primers with different proportions of primers in the set to provide balanced amplicon read depth.

Abstract: An assay provides comprehensive genomic profiling for plasma-derived circulating tumor DNA from solid tumors. The assay covers genes for mutations including single nucleotide variants (SNVs), insertions and deletions (indels), copy number variants (CNVs), and fusions, as well as microsatellite instability (MSI) status. The assay may use a custom hybrid capture process with optimized chemistry and panel design, as well as novel algorithms for SNV, indel, and CNV data filtering to optimize performance.

Abstract: The present disclosure provides a vaccine composition comprising six or more homologous distinct antigen components. Any two of the six or more homologous distinct antigen components may share less than 98% or 95% sequence identity. The homologous distinct antigen components may comprise proteins. An amount of a protein in a dose of said human adult vaccine composition may be about 600 nanograms (ng) to about 3 micrograms (?g). The homologous distinct antigen components may comprise a plurality of RNA. An amount of an RNA of said plurality of RNA in a dose of said vaccine composition may be about 1 ng to about 5 ?g per dose. The homologous distinct antigen components may comprise a plurality of proteins displayed on heterologous viral-like particles (VLPs). An amount of a protein displayed on a heterologous VLP of the plurality of proteins displayed on heterologous VLPs in a dose of the vaccine composition may be about 1 ng to about 5 ?g per dose.

Abstract: An assay provides comprehensive genomic profiling for plasma-derived circulating tumor DNA from solid tumors. The assay covers genes for mutations including single nucleotide variants (SNVs), insertions and deletions (indels), copy number variants (CNVs), and fusions, as well as microsatellite instability (MSI) status. The assay may use a custom hybrid capture process with optimized chemistry and panel design, as well as novel algorithms for SNV, indel, and CNV data filtering to optimize performance.

Abstract: Provided herein are methods and systems for cell-free DNA extraction from liquid biological samples. The methods can be employed for determination of fetal DNA fraction and non- invasive prenatal screening of fetal aneuploidies and analyses of other types of cell-free DNA.

Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.

Abstract: Embodiments of a method and/or system can include generating a set of target-associated molecules (e.g., spike-in molecules) associated with one or more biological targets; generating one or more spike-in mixtures based on processing the set of target-associated molecules with one or more samples including the one or more biological targets; performing one or more Sanger sequencing operations on the one or more spike-in mixtures; determining one or more abundance metrics based on chromatogram-related outputs from the one or more Sanger sequencing operations; and/or facilitating characterization of one or more medical conditions based on the one or more abundance metrics.

Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.

Abstract: Embodiments of a method for accurate determination of biological target abundance can include generating a first set of molecules associated with a target sequence, where the first set of molecules includes a first set of dilution tags associated with a relative concentration profile; generating a second set of molecules including a second set of dilution tags associated with the first set of dilution tags; generating a dilution tagged mixture; amplifying the subsets of dilution tagged genetic targets using the second set of molecules; generating a modified dilution tagged mixture from the amplified subsets; determining, for the biological sample, a count of the distinct molecules including the target sequence; and/or determining, for the biological sample, an assessment of relative concentrations distinct species, such as over a vast dynamic range.

Abstract: Provided herein are methods and systems for cell-free DNA extraction from liquid biological samples. The methods can be employed for determination of fetal DNA fraction and non-invasive prenatal screening of fetal aneuploidies and analyses of other types of cell-free DNA.

Abstract: Methods to quantify methylation in a DNA sample include treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules (e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, and determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.

Abstract: This disclosure provides antibodies and antigen-binding fragments that can be administered to a subject that has been bitten by a venomous snake. Antibodies and antigen-binding fragments herein can be capable of treating or curing the subject and may provide protection against snake venom for up to several weeks. A combination or population of antibodies and antigen-binding fragments can be administered to the subject where the type of snake is not known or where a subject has been bitten by more than one species of snake. This disclosure further provides methods for identification of such broadly-neutralizing antibodies.

Abstract: Embodiments of a method for accurate determination of biological target abundance can include generating a first set of molecules associated with a target sequence, where the first set of molecules includes a first set of dilution tags associated with a relative concentration profile; generating a second set of molecules including a second set of dilution tags associated with the first set of dilution tags; generating a dilution tagged mixture; amplifying the subsets of dilution tagged genetic targets using the second set of molecules; generating a modified dilution tagged mixture from the amplified subsets; determining, for the biological sample, a count of the distinct molecules including the target sequence; and/or determining, for the biological sample, an assessment of relative concentrations distinct species, such as over a vast dynamic range.

Abstract: A hashing system (e.g., including a message expander) receives word data associated with a message. The hashing system identifies a plurality of message expansion computations that are configured to use the word data to generate a hash for the message. The hashing system pre-computes at least one message expansion computation of the plurality of message expansion computations using the word data to generate at least one constant. The hashing system executes a modified plurality of message expansion computations to generate the hash of the message. Executing the modified plurality of message expansion computations includes replacing the at least one message expansion computation of the plurality of message expansion computations with the at least one constant.

Abstract: Methods to quantify methylation in a DNA sample include treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules (e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, and determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.

Abstract: A hashing system (e.g., including a message expander) receives word data associated with a message. The hashing system identifies a plurality of message expansion computations that are configured to use the word data to generate a hash for the message. The hashing system pre-computes at least one message expansion computation of the plurality of message expansion computations using the word data to generate at least one constant. The hashing system executes a modified plurality of message expansion computations to generate the hash of the message. Executing the modified plurality of message expansion computations includes replacing the at least one message expansion computation of the plurality of message expansion computations with the at least one constant.

Abstract: Aspects of the present disclosure includes methods to quantify methylation in a DNA sample, where the method includes treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.

Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.

Abstract: Provided herein are methods and systems for cell-free DNA extraction from liquid biological samples. The methods can be employed for determination of fetal DNA fraction and non-invasive prenatal screening of fetal aneuploidies and analyses of other types of cell-free DNA.