Patents by Inventor David V. Thompson

David V. Thompson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD TO ENHANCE SCREENING FOR HOMOLOGOUS RECOMBINATION IN GENOME EDITED CELLS USING RECOMBINATION-ACTIVATED FLUORESCENT DONOR DELIVERY VECTOR
    Publication number: 20200157531
    Abstract: Constructs, vectors, and methods for enhancing homology directed repair using the CRISPR/Cas9 gene editing platform are disclosed.
    Type: Application
    Filed: November 14, 2019
    Publication date: May 21, 2020
    Inventors: David V. Thompson, Mark E. Burkard
  • Vector for in vitro mutagenesis and use thereof
    Patent number: 5955363
    Abstract: Site-directed in vitro mutagenesis is obtained utilizing a specially-engineered plasmid vector. The vector is engineered to contain an inactivated genetic marker which marker is capable of being reverted to functional expression. Additionally, the vector contains a second genetic marker, a polylinker region and an f1 replication origin.
    Type: Grant
    Filed: May 17, 1993
    Date of Patent: September 21, 1999
    Assignee: Promega Corporation
    Inventors: Martin K. Lewis, David V. Thompson
  • Compositions for cytochrome P450 biotransformation reactions
    Patent number: 5891696
    Abstract: A storable biotransformation mixture that can oxidize a drug, comprising a cytochrome P450 enzyme, an electron donor; and, a buffer solution. In a preferred embodiment the mixture also contains liposomes, cytochrome b.sub.5 and NADPH-P450 reductase as the electron donor. The mixture is storable at a temperature less than 8.degree. C. to for at least two weeks and is able to oxidize a drug after storage.
    Type: Grant
    Filed: August 1, 1997
    Date of Patent: April 6, 1999
    Assignee: Panvera Corporation
    Inventors: Peter M. Shaw, Robert G. Lowery, David V. Thompson
  • Method and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tag
    Patent number: 5786139
    Abstract: The present invention is based on the degradation of a fluorescence labeled nucleic acid such as DNA (F-DNA), RNA (F-RNA) or DNA/RNA substrate. The label may be covalently attached to the relatively high volume substrate at one position or many positions. The nucleic acid is then put in contact with another substance and activity is detected by Fluorescence Polarization.
    Type: Grant
    Filed: December 9, 1994
    Date of Patent: July 28, 1998
    Assignee: PanVera Corporation
    Inventors: Thomas J. Burke, Randall E. Bolger, William J. Checovich, David V. Thompson
  • Coupled transcription and translation in eukaryotic cell-free extract
    Patent number: 5492817
    Abstract: A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.
    Type: Grant
    Filed: June 27, 1994
    Date of Patent: February 20, 1996
    Assignee: Promega Corporation
    Inventors: David V. Thompson, Thomas R. Van Oosbree, Gregory S. Beckler, John F. Van Herwynen
  • Coupled transcription and translation in eukaryotic cell-free extract
    Patent number: 5324637
    Abstract: A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.
    Type: Grant
    Filed: November 9, 1993
    Date of Patent: June 28, 1994
    Assignee: Promega Corporation
    Inventors: David V. Thompson, Thomas R. Van Oosbree
  • Method of using a TAQ DNA polymerase without 5'-3'-exonuclease activity
    Patent number: 5108892
    Abstract: The present invention is directed to a modified Taq DNA polymerase and methods for its use.
    Type: Grant
    Filed: August 3, 1989
    Date of Patent: April 28, 1992
    Assignee: Promega Corporation
    Inventors: Thomas J. Burke, David V. Thompson, Sandra L. Spurgeon