Patents by Inventor David Valenzuela
David Valenzuela has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240157057Abstract: A real-time drug injection time duration measurement system includes a sound transducer, a waveform shaping circuit operably coupled therewith, a pulse clamping circuit operably coupled with the waveform shaping circuit, and a timer operably coupled with the pulse clamping circuit. The sound transducer detects a first sound associated with drug delivery initiation and a second sound associated with drug delivery completion and is further converts the detected first and second sounds into a first electronic waveform and a second electronic waveform, respectively. The waveform shaping circuit converts the first and second waveforms into a first pulse and a second pulse, respectively. The pulse clamping circuit triggers upon receiving the first pulse and generate a regenerated pulse. The timer initiates upon receiving the regenerated pulse and stop upon receiving a subsequent regenerated pulse via the pulse clamping circuit.Type: ApplicationFiled: March 28, 2022Publication date: May 16, 2024Applicant: AMGEN INC.Inventors: Huixing Jin, Julian JAZAYERI, Sergio Giovanni VALENZUELA, Ryan David SELVE, Sivakumar PARAMANANDAM
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Publication number: 20230211042Abstract: This disclosure relates to pectin-based polymer compositions and methods of use thereof to cover, protect, and seal injuries, e.g., surgical wounds, in a mesothelial tissue. The methods include obtaining a bioadhesive pectin-based polymer composition including a complex of high-methoxyl pectin (HMP) and carboxymethylcellulose (CMC) in a ratio from about 10 to 1 to 1 to 10 by weight; applying the composition to an injured mesothelial tissue; and applying pressure for at least one minute to enable the composition to bind to the mesothelial tissue.Type: ApplicationFiled: December 23, 2022Publication date: July 6, 2023Inventors: Steven James Mentzer, Cristian David Valenzuela, Alexandra Brooke Ysasi, Andrew Barrett Servais
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Patent number: 11534524Abstract: This disclosure relates to pectin-based polymer compositions and methods of use thereof to cover, protect, and seal injuries, e.g., surgical wounds, in a mesothelial tissue. The methods include obtaining a bioadhesive pectin-based polymer composition including a complex of high-methoxyl pectin (HMP) and carboxymethylcellulose (CMC) in a ratio from about 10 to 1 to 1 to 10 by weight; applying the composition to an injured mesothelial tissue; and applying pressure for at least one minute to enable the composition to bind to the mesothelial tissue.Type: GrantFiled: February 1, 2021Date of Patent: December 27, 2022Assignee: The Brigham and Women's Hospital, Inc.Inventors: Steven James Mentzer, Cristian David Valenzuela, Alexandra Brooke Ysasi, Andrew Barrett Servais
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Publication number: 20210154354Abstract: This disclosure relates to pectin-based polymer compositions and methods of use thereof to cover, protect, and seal injuries, e.g., surgical wounds, in a mesothelial tissue. The methods include obtaining a bioadhesive pectin-based polymer composition including a complex of high-methoxyl pectin (HMP) and carboxymethylcellulose (CMC) in a ratio from about 10 to 1 to 1 to 10 by weight; applying the composition to an injured mesothelial tissue; and applying pressure for at least one minute to enable the composition to bind to the mesothelial tissue.Type: ApplicationFiled: February 1, 2021Publication date: May 27, 2021Inventors: Steven James Mentzer, Cristian David Valenzuela, Alexandra Brooke Ysasi, Andrew Barrett Servais
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Patent number: 10912858Abstract: This disclosure relates to pectin-based polymer compositions and methods of use thereof to cover, protect, and seal injuries, e.g., surgical wounds, in a mesothelial tissue. The methods include obtaining a bioadhesive pectin-based polymer composition including a complex of high-methoxyl pectin (HMP) and carboxymethylcellulose (CMC) in a ratio from about 10 to 1 to 1 to 10 by weight; applying the composition to an injured mesothelial tissue; and applying pressure for at least one minute to enable the composition to bind to the mesothelial tissue.Type: GrantFiled: April 30, 2018Date of Patent: February 9, 2021Assignee: The Brigham and Women's Hospital, Inc.Inventors: Steven James Mentzer, Cristian David Valenzuela, Alexandra Brooke Ysasi, Andrew Barrett Servais
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Publication number: 20210024953Abstract: Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.Type: ApplicationFiled: August 31, 2020Publication date: January 28, 2021Applicant: Regeneron Pharmaceuticals, Inc.Inventors: David Frendewey, Gustavo Droguett, Anthony Gagliardi, Junko Kuno, Wojtek Auerbach, David Valenzuela
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Patent number: 10793874Abstract: Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.Type: GrantFiled: February 22, 2018Date of Patent: October 6, 2020Assignee: Regeneron Pharmaceuticals, Inc.Inventors: David Frendewey, Gustavo Droguett, Anthony Gagliardi, Junko Kuno, Wojtek Auerbach, David Valenzuela
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Publication number: 20180311403Abstract: This disclosure relates to pectin-based polymer compositions and methods of use thereof to cover, protect, and seal injuries, e.g., surgical wounds, in a mesothelial tissue. The methods include obtaining a bioadhesive pectin-based polymer composition including a complex of high-methoxyl pectin (HMP) and carboxymethylcellulose (CMC) in a ratio from about 10 to 1 to 1 to 10 by weight; applying the composition to an injured mesothelial tissue; and applying pressure for at least one minute to enable the composition to bind to the mesothelial tissue.Type: ApplicationFiled: April 30, 2018Publication date: November 1, 2018Inventors: Steven James Mentzer, Cristian David Valenzuela, Alexandra Brooke Ysasi, Andrew Barrett Servais
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Patent number: 9885058Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: GrantFiled: April 12, 2017Date of Patent: February 6, 2018Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20170218399Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Patent number: 9655351Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: GrantFiled: June 22, 2016Date of Patent: May 23, 2017Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20160295841Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: ApplicationFiled: June 22, 2016Publication date: October 13, 2016Applicant: Regeneron Pharmaceuticals, Inc.Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Patent number: 9398762Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: GrantFiled: November 11, 2014Date of Patent: July 26, 2016Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Patent number: 9149026Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: GrantFiled: June 10, 2011Date of Patent: October 6, 2015Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Wojtek Auerbach, Thomas Dechiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20150067901Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: ApplicationFiled: November 11, 2014Publication date: March 5, 2015Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20110307968Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: ApplicationFiled: June 10, 2011Publication date: December 15, 2011Applicant: Regeneron Pharmaceuticals, Inc.Inventors: Wojtek AUERBACH, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20080078000Abstract: Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.Type: ApplicationFiled: September 27, 2007Publication date: March 27, 2008Applicant: Regeneron Pharmaceuticals, Inc.Inventors: William Poueymirou, Thomas Dechiara, Wojtek Auerbach, David Frendewey, David Valenzuela
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Publication number: 20080078001Abstract: Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.Type: ApplicationFiled: September 27, 2007Publication date: March 27, 2008Applicant: Regeneron Pharmaceuticals, Inc.Inventors: William Poueymirou, Thomas Dechiara, Wojtek Auerbach, David Frendewey, David Valenzuela
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Publication number: 20080028479Abstract: Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.Type: ApplicationFiled: September 27, 2007Publication date: January 31, 2008Applicant: Regeneron Pharmaceuticals, Inc.Inventors: William Poueymirou, Thomas Dechiara, Wojtek Auerbach, David Frendewey, David Valenzuela
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Publication number: 20070275466Abstract: Methods of creating mutations in genomic exons by inserting introns into the genomic exons via homologous recombination. Also, methods are provided for introducing modifications into genomic exons by inserting introns into the genomic exons via homologous recombination such that a mature mRNA transcript produced from a genomic region of the genome comprising the genomic exon does not contain the modification are provided. The methods provide for a rapid method for introducing mutations and/or modifications of any type into a mammalian cell genome.Type: ApplicationFiled: April 17, 2007Publication date: November 29, 2007Inventors: Aris Economides, David Valenzuela, Samuel Davis, George Yancopoulos