Abstract: The present invention provides for methods of treating diabetes by providing to a subject brown adipose tissue. The tissue may be transplanted from a culture of the subject's own tissue (autograft) or from a donor. These methods can achieve normoglycemia and suppress hypergluconemia, and alleviate conditions associated with such, even in the absence of or at extremely low levels of adjunct insulin therapy, and without any appreciable increase in insulinogenesis.

Abstract: The present invention relates to providing novel therapeutics for treating diabetes other glycemic disorders. Such therapeutics involve the signaling pathways that contribute to regulation of glucose-stimulated insulin secretion. Of particular interest are modulators of a key component in the glucokinase pathway. Thus, the present provides methods of screening for modulators of glucokinase activity, expression, translocation, conformation, nitrosylation and interaction with other molecules as useful target for pharmacological manipulation in the treatment of diabetes and other glycemic disorders.

Abstract: A digital frequency domain fluorometer utilizing a computer-controlled digital data acquisition system is use for the study of fluorescence and phosphorescence phenomena in the bio-chemical, biological and bio-physics arts. The computer is used for the direct collection of data, as well as for the filtering and calculation of the phase and modulation values of the sample under study. From these values, fluorescence lifetimes and anisotropy decay times can determined. The digital data acquisition system provides for the simultaneous collection and processing of several modulation frequencies. In addition, the digital frequency domain fluorometer can utilize an array detector for detecting the modulated light from the various samples under study. The use of the array detector provides a means for independently collecting data over a large number of pixels. This configuration allows for a time resolved image to be collected at once.