Abstract: Embodiments of the present disclosure provide techniques for using an inverted index in a pipelined search query. A field searchable data store is provided that comprises a plurality of event records, each event record comprising a time-stamped portion of raw machine data. Responsive to the reciept of an incoming search query, the search engine accesses an inverted index, wherein each entry in the inverted index comprises at least one field name, a corresponding at least one field value and a reference value associated with each field name and value pair that identifies a location in the data store where an associated event record is stored. Once the inverted index is accessed, it can be used to identify and search a subset of the plurality of event records, wherein the subset comprises one or more event records with corresponding reference values in the inverted index.

Abstract: This invention describes a method of controlling the hybridization yield of nucleic acid probes by adjusting the relative concentrations of auxiliary oligonucleotides to the probes and the targets. The auxiliary oligonucleotide is partially or fully complementary to either the probe or the target, and is released upon hybridization of the probe to the target.

Abstract: Provided herein are high-throughput methods for genetic barcoding and analysis, e.g., for tagging each biomaterial apsule with a barcode cell. These barcode cells are derived from patient samples, and thus embody natural human genetic variation. Also provided are SNP panels that can be used as genetic barcodes to identify the identity of a cell.

Abstract: A gas engine is disclosed. The gas engine may include a combustion cylinder. The combustion cylinder may include an intake with an intake valve. The combustion cylinder may include an exhaust with an exhaust valve. The gas engine may include a gas admission valve assembly coupled to the intake. The gas admission assembly may include a trimmable electro-hydraulically actuatable gas admission valve to control a gas flow into the intake.

Abstract: Provided herein are compositions and methods for assembling multiple DNA molecules into a linear concatemer, with applications to nanopore sequencing of DNA sequence variations.

Abstract: The present disclosure relates to implantable constructs designed to deliver antigenic therapeutic reagents to a subject while providing protection from host immune responses. In certain aspects, the constructs are designed to degrade over time or upon a particular signal, thereby providing control of the length of time the therapeutic agent is delivered to the subject.

Abstract: The present invention provides an oligonucleotide composition including a blocker and a first primer oligonucleotide. The blocker oligonucleotide includes a first sequence having a target-neutral subsequence and a blocker variable subsequence. The non-target specific subsequence is flanked on its 3? and 5? ends by the target-neutral subsequence and is continuous with the target-neutral subsequence. The first primer oligonucleotide is sufficient to induce enzymatic extension; herein the first primer oligonucleotide includes a second sequence. The second sequence overlaps with the 5? end of the target-neutral subsequence by at least 5 nucleotides; herein the second sequence includes an overlapping subsequence and a non-overlapping subsequence. The second sequence does not include the non-target specific subsequence.

Abstract: The disclosure describes apparatus and methods for multiplexed amplification and detection of nucleic acid targets in a sample. Embodiments of the present disclosure include a mechanical system configured to provide loading, vertical positioning and clamping of a chip; a thermal control system configured to maintain distinct temperatures of the chip, and an optical fluorescence imaging system.

Abstract: Provided herein are methods for quantifying foreign cell-free DNA (cfDNA) via SNP profiling of low-volume blood sample. The methods allow for monitoring the status of organ transplant rejection through analysis of small volumes of patient capillary blood samples collected non-invasively with fingersticks or other devices. The methods also allow for guiding the dosage of immunosuppressant and for preparing for a new organ transplant in case of imminent organ failure.

Abstract: A gaseous fuel mixer assembly for an engine includes a mixer housing forming gas delivery openings, and positioned to extend across a flow path formed by an intake conduit for the engine. A spool valve is within a central bore in the mixer housing and includes gas distribution openings selectively connectable to the gas delivery openings by moving the spool valve within the mixer housing using a piezoelectric actuator coupled with the spool valve by way of a pivot arm. Sealing lands of the spool valve are in an alternating arrangement with the gas distribution openings, such that at the closed position the sealing lands block the gas distribution openings from the gas delivery openings, and at the open position the respective openings are fluidly connected.

Abstract: A gaseous fuel mixer assembly for an engine includes a mixer housing forming gas delivery openings, and positioned to extend across a flow path formed by an intake conduit for the engine. A spool valve is within a central bore in the mixer housing and includes gas distribution openings selectively connectable to the gas delivery openings by moving the spool valve within the mixer housing using a piezoelectric actuator coupled with the spool valve by way of a pivot arm. Sealing lands of the spool valve are in an alternating arrangement with the gas distribution openings, such that at the closed position the sealing lands block the gas distribution openings from the gas delivery openings, and at the open position the respective openings are fluidly connected.

Abstract: Provided herein are primers and primer systems having improved specificity and kinetics over existing primers, and methods of use thereof.

Abstract: The present disclosure describes the thermodynamic design and concentrations necessary to design probe compositions with desired optimal specificity that enable enrichment, detection, quantitation, purification, imaging, and amplification of rare-allele-bearing species of nucleic acids (prevalence <1%) in a large stoichiometric excess of a dominant-allele-bearing species (wildtype). Being an enzyme-free and homogeneous nucleic acid enrichment composition, this technology is broadly compatible with nearly all nucleic acid-based biotechnology, including plate reader and fluorimeter readout of nucleic acids, microarrays, PCR and other enzymatic amplification reactions, fluorescence barcoding, nanoparticle-based purification and quantitation, and in situ hybridization imaging technologies.

Abstract: The present disclosure describes the thermodynamic design and concentrations necessary to design probe compositions with desired optimal specificity that enable enrichment, detection, quantitation, purification, imaging, and amplification of rare-allele-bearing species of nucleic acids (prevalence <1%) in a large stoichiometric excess of a dominant-allele-bearing species (wildtype). Being an enzyme-free and homogeneous nucleic acid enrichment composition, this technology is broadly compatible with nearly all nucleic acid-based biotechnology, including plate reader and fluorimeter readout of nucleic acids, microarrays, PCR and other enzymatic amplification reactions, fluorescence barcoding, nanoparticle-based purification and quantitation, and in situ hybridization imaging technologies.

Abstract: Operating an internal combustion engine system includes advancing a prechamber piston in a fuel delivery igniter to push a main charge of fuel from a prechamber into a cylinder, and advancing the prechamber piston to compression-ignite a pilot charge within the prechamber. Combustion gases of the pilot charge are conveyed into the cylinder to ignite the main charge. Operation of the engine system provides sparkless gaseous fuel ignition.

Abstract: An ignition system for a gaseous fuel engine includes an igniter and an actuator structured to apply an actuating force to a piston within the igniter, to autoignite an ignition charge of fuel and air within the igniter. A housing of the igniter includes a gas orifice having a flow area that is increased between a combustion pre-chamber in the igniter and a main combustion chamber in the engine, to limit velocity of outgoing combustion gases to below a threshold velocity for engine mis-fire.

Abstract: Compositions and methods for highly specific nucleic acid probes and primers are provided. The probe system comprises a complement strand and a protector stand that form a partially double-stranded probe. The reaction standard free energy of hybridization between the probe and target nucleic acid as determined by Expression 1 (?G°rxn=?G°t-TC??G°nh-PC+(?G°v-TC??G°h-PC)) is from about ?4 kcal/mol to about +4 kcal/mol. Alternatively, the reaction standard free energy of hybridization between the probe and target nucleic acid is determined by Expression 1 to be within 5 kcal/mol of the standard free energy as determined by Expression 2 (?R?ln(([P]0?[C]0)/[C]0)]), where the [P]0 term of Expression 2 equals the concentration of the protector strand and the [C]0 term of Expression 2 equals the concentration of the complement strand. In addition, a method for on-the-fly fine tuning of a reaction using the present probe is provided.

Abstract: This invention describes a method of controlling the hybridization yield of nucleic acid probes by adjusting the relative concentrations of auxiliary oligonucleotides to the probes and the targets. The auxiliary oligonucleotide is partially or fully complementary to either the probe or the target, and is released upon hybridization of the probe to the target.

Abstract: Provided herein are methods for encoding information in DNA molecules in a way that allows rapid and permanent erasure of information. As such, methods of erasing such information are also provided. Also provided are compositions that so encode information.

Abstract: Provided herein are reagents and methods for simultaneously enriching many potential rare genetic variants at different genetic loci. The rare variants enriched can include single nucleotide polymorphisms (SNPs), single nucleotide variants, or small insertions and deletions. Embodiments of the invention include procedures for integration with downstream next generation sequencing (NGS) analysis. Embodiments of the invention include analysis of nonpathogenic SNPs for the determination of cell identity and detection of cell contamination using qPCR or NGS.