Patents by Inventor David Zelmanovic
David Zelmanovic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230176035Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising at least one of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring at least one of an amount of light absorbed by the RBCs to obtain an RBC absorption value, an amount of light scattered by WBCs to obtain a WBC scatter value, and an amount of light scattered by PLTs to obtain a PLT scatter value; and determining a concentration of at least one of the RBCs, WBCs, and PLTs present in the sample mixture.Type: ApplicationFiled: January 17, 2023Publication date: June 8, 2023Inventors: David ZELMANOVIC, John ROCHE
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Patent number: 11579139Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising at least one of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring at least one of an amount of light absorbed by the RBCs to obtain an RBC absorption value, an amount of light scattered by WBCs to obtain a WBC scatter value, and an amount of light scattered by PLTs to obtain a PLT scatter value; and determining a concentration of at least one of the RBCs, WBCs, and PLTs present in the sample mixture.Type: GrantFiled: December 22, 2021Date of Patent: February 14, 2023Assignee: LabThroughput LLCInventors: David Zelmanovic, John Roche
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Publication number: 20220170907Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising at least one of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring at least one of an amount of light absorbed by the RBCs to obtain an RBC absorption value, an amount of light scattered by WBCs to obtain a WBC scatter value, and an amount of light scattered by PLTs to obtain a PLT scatter value; and determining a concentration of at least one of the RBCs, WBCs, and PLTs present in the sample mixture.Type: ApplicationFiled: December 22, 2021Publication date: June 2, 2022Inventors: David ZELMANOVIC, John ROCHE
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Publication number: 20200116698Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring an amount of light absorbed by the RBCs; measuring an amount of light scattered by WBCs, and PLTs; determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.Type: ApplicationFiled: December 13, 2019Publication date: April 16, 2020Inventors: David ZELMANOVIC, John ROCHE
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Patent number: 10509024Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring an amount of light absorbed by the RBCs; measuring an amount of light scattered by WBCs, and PLTs; determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.Type: GrantFiled: May 3, 2017Date of Patent: December 17, 2019Assignee: LABTHROUGHPUT LLCInventors: David Zelmanovic, John Roche
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Publication number: 20170322198Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring an amount of light absorbed by the RBCs; measuring an amount of light scattered by WBCs, and PLTs; determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.Type: ApplicationFiled: May 3, 2017Publication date: November 9, 2017Inventors: David ZELMANOVIC, John ROCHE
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Patent number: 7745219Abstract: The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.Type: GrantFiled: July 21, 2004Date of Patent: June 29, 2010Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Michael J. Sommer, Pasquale Degiorgio, Bronislaw P. Czech, Gena Fischer, E. Sabrinah Chapman, David Zelmanovic, Jolanta Kunicka
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Patent number: 7670798Abstract: The present invention describes semi- and fully-automated methods and reagents therefor for the assay and analysis of body fluid samples, particularly non-blood samples. The methods and reagents are especially useful for the assay and analysis of cerebrospinal fluid (CSF) samples. The reagent compositions sphere and fix all cells in the sample in suspension. Reported results can include red blood cell (RBC) and white blood cell (WBC) counts, WBC differential values, cell-by-cell volumes and dry-mass concentrations.Type: GrantFiled: May 20, 2003Date of Patent: March 2, 2010Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Leonard Ornstein, Gena Fischer, David Zelmanovic, Pamela Elsins, Jolanta Kunicka, Michael J. Malin
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Publication number: 20050026240Abstract: The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.Type: ApplicationFiled: July 21, 2004Publication date: February 3, 2005Inventors: Michael Sommer, Pasquale Degiorgio, Bronislaw Czech, Gena Fischer, E. Chapman, David Zelmanovic, Jolanta Kunicka
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Publication number: 20030215890Abstract: The present invention describes semi- and fully-automated methods and reagents therefor for the assay and analysis of body fluid samples, particularly non-blood samples. The methods and reagents are especially useful for the assay and analysis of cerebrospinal fluid (CSF) samples. The reagent compositions sphere and fix all cells in the sample in suspension. Reported results can include red blood cell (RBC) and white blood cell (WBC) counts, WBC differential values, cell-by-cell volumes and dry-mass concentrations.Type: ApplicationFiled: May 20, 2003Publication date: November 20, 2003Inventors: Leonard Ornstein, Gena Fischer, David Zelmanovic, Pamela Elsins, Jolanta Kunicka, Michael J. Malin
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Patent number: 6524858Abstract: The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of the light scattered and absorbed by each cell. The single channel utilized in the method was previously known and used only for red blood cell and reticulocyte analysis. The method involves the use of an organic dye in the reagent solution for staining the nucleic acid of reticulocytes, including reticulated platelets, and white blood cells in the sample. The single channel method developed and described is particularly useful for determining white blood cell counts and assessing parameters of a whole blood sample, for blood samples from both human and non-human mammals.Type: GrantFiled: March 31, 2000Date of Patent: February 25, 2003Assignee: Bayer CorporationInventors: David Zelmanovic, Valentine Jones
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Patent number: 6521729Abstract: The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles.Type: GrantFiled: November 2, 1999Date of Patent: February 18, 2003Assignee: Bayer CorporationInventors: David Zelmanovic, William Moskalski, Ronny DeClercq, Frank Louwet, Eberhard Kuckert, Wolfgang Podszun
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Patent number: 6114173Abstract: The invention provides a very fast and reliable method and reagent composition for determining reticulocyte and erythrocyte counts, identification and characterization, as well as platelet counts, in a whole blood sample using fully automated hematology analyzers. The reagent composition includes a zwitterionic surfactant as sphering agent for reticulocytes and erythrocytes, an organic cationic dye, e.g., Oxazine 750, for staining the reticulocytes, and buffer solution components for maintaining a reagent pH of about 7.2 to 7.8, and an osmolarity of about 292.+-.5. On the basis of the particular pH, ionic strength, and dye concentration, the reagent composition of the present method improves upon previous methods, thus allowing fully automated blood sample analyses to be completed in less than about 30 seconds, with only about a 20 second incubation of sample in the reagent solution before analysis.Type: GrantFiled: April 3, 1997Date of Patent: September 5, 2000Assignee: Bayer CorporationInventors: David Zelmanovic, Lynn Paseltiner, Martin Sorette
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Patent number: 6074879Abstract: The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles.Type: GrantFiled: June 17, 1998Date of Patent: June 13, 2000Assignee: Bayer CorporationInventors: David Zelmanovic, William Moskalski, Ronny DeClercq, Frank Louwet, Eberhard Kuckert, Wolfgang Podszun
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Patent number: 6025201Abstract: The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do non-activated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.Type: GrantFiled: June 27, 1997Date of Patent: February 15, 2000Assignee: Bayer CorporationInventors: David Zelmanovic, Gregory M. Colella, Edward J. Hetherington, Evelyn Sabrinah Chapman, Lynn Paseltiner
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Patent number: 5817519Abstract: The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do unactivated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.Type: GrantFiled: November 1, 1996Date of Patent: October 6, 1998Assignee: Bayer CorporationInventors: David Zelmanovic, Gregory M. Colella, Edward J. Hetherington, Evelyn Sabrinah Chapman, Lynn Paseltiner
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Patent number: 4677298Abstract: In a lithographic printing system, a light source and a plurality of photodetectors are used to monitor the amount of fountain solution interacting with the ink. The light source irradiates an ink form roller and the photodetectors are disposed at a specular angle and at one or more non-specular angles to monitor the reflected light. An electronic circuit is responsive to the output of the photodetectors and is used for providing an indication of the amount of fountain solution emulsified in the ink, and lying on the surface of the ink.Type: GrantFiled: June 7, 1984Date of Patent: June 30, 1987Assignee: Kollmorgen Technologies CorporationInventors: David Zelmanovic, Stanley J. Kishner
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Patent number: 4631408Abstract: In the manufacture of a multilayer polymer film, the thickness and degree of orientation are simultaneously measured. A beam of infrared radiation having predetermined wavelengths is either transmitted through or reflected by the polymer film to be measured and received by a photodetector. The photodetector provides an electrical output signal which can be analyzed to determine the thickness and degree of orientation.Type: GrantFiled: September 24, 1984Date of Patent: December 23, 1986Assignee: Kollmorgen Technologies CorporationInventors: David Zelmanovic, Stanley J. Kishner