Abstract: The invention provides methods for diagnosing disease by detecting extracellular vesicle-derived markers in combination with other diagnostic and screening assays. The methods include, but are not limited to, the analysis of extracellular vesicle-derived biomarkers obtained from bodily fluid samples. The methods include, but are not limited to, diagnostic and screening assays, including medical imaging. The present disclosure in one aspect provides technologies for detection and/or screening of a plurality of cancers. In another aspect, technologies provided herein are useful for selecting and/or monitoring and/or evaluating efficacy of, a treatment administered to a subject determined to have or susceptible to cancer.

Abstract: The invention provides methods and kits for the analysis of extracellular vesicle-derived biomarkers obtained from dried body fluid samples; including but not limited to blood spots extracted onto or into absorptive surfaces for analysis of extracellular vesicles for disease diagnosis, staging, monitoring and selection and efficacy of therapeutics.

Abstract: The present disclosure relates to methods and compositions, e.g., kits, for quantitatively detecting an antibody of a subject to an infectious organism. In some embodiments, the present disclosure provides for methods and compositions, e.g., kits, for quantitatively detecting a human antibody to SARS-CoV-2 polypeptide or S (spike) polypeptide. Certain applications and uses of the present methods and compositions, e.g., kits, are also provided.

Abstract: The present disclosure relates to methods and compositions, e.g., kits, for assessing or detecting SARS-CoV-2 antigen(s), e.g., SARS-CoV-2 nucleocapsid protein (N-protein), in a sample or a blood sample, e.g., serum, plasma, dried blood spots (DBS) and/or a saliva sample. Certain applications and uses of the present methods and compositions, e.g., kits, are also provided.

Abstract: The present disclosure relates to methods and compositions, e.g., kits, for quantitatively detecting an antibody of a subject to an infectious organism. In some embodiments, the present disclosure provides for methods and compositions, e.g., kits, for quantitatively detecting a human antibody to SARS-CoV-2 polypeptide or S (spike) polypeptide. Certain applications and uses of the present methods and compositions, e.g., kits, are also provided.