Patents by Inventor Dawson Wong

Dawson Wong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Molecular analysis using a magnetic sifter and nanowell system
    Patent number: 10167515
    Abstract: A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: January 1, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Seung-min Park, Dawson Wong, Chin Chun Ooi, Sanjiv Sam Gambhir, Viswam S. Nair, Shan X. Wang
  • Molecular Analysis using a Magnetic Sifter and Nanowell System
    Publication number: 20180002760
    Abstract: A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
    Type: Application
    Filed: April 20, 2016
    Publication date: January 4, 2018
    Inventors: Seung-min Park, Dawson Wong, Chin Chun Ooi, Sanjiv Sam Gambhir, Viswam S. Nair, Shan X. Wang