Abstract: The present invention relates to methods for determining the quantity of an analyte in sample using lateral flow strips comprising highly-doped upconversion nanoparticles.

Abstract: Methods are presented for tuning the time-domain emissive profile of single upconversion nanoparticles using a number of different techniques so as to increase the coding capacity at the nanoscale. The disclosure also relates to time-resolved wide-field imaging and deep-learning techniques to decode the nanoparticle fingerprints.

Abstract: The present invention relates to methods for determining the quantity of an analyte in sample using lateral flow strips comprising highly-doped upconversion nanoparticles.

Abstract: A system for microscopy comprising a reflector for a sample to be disposed adjacent thereto. The system comprises a radiation source for generating electromagnetic radiation and arranged for the electromagnetic radiation when so generated to pass at least partially through the sample when so disposed. When the electromagnetic radiation is so generated, interference is localized at least partially within the sample when so positioned, the interference being electromagnetic interference between the electromagnetic radiation and a reflected electromagnetic radiation that is at least part of the electromagnetic radiation reflected from the reflector.

Abstract: A system, device and/or method for multiplex assays. In a particular, but non-limiting, example there is provided a multiplex array, such as a suspension array. Luminescence decay lifetimes are utilized for probes in a suspension array, and coding/decoding the codes from time-resolved spectra. Lifetime populations can be generated at distinct color bands. A novel temporal technique or dimension is applied over conventional spectral and intensity combinations, thereby expanding the multiplexing capacity of a suspension array. In one example form, the multiplexing capacity of a suspension array can be expanded to the order of about 58. This provides a reliable, high-throughput and relatively inexpensive solution for multiplex assays in various areas of application such as life sciences, data storage and security.

Abstract: Disclosed is a method for enhancing upconversion luminescence of rare-earth doped particles comprising a host material, an enriched concentration of activator (emitter) and a sufficient concentration level of sensitiser, the method comprising subjecting the particles to increased irradiance. The increased irradiance is higher than presently used relatively low irradiance levels. Enhancing upconversion luminescence involves enhancing luminescence intensity, brightness and/or upconversion efficiency. Particles are preferably subjected to an irradiance power density sufficient to overcome or reverse concentration quenching. The activator preferably has an intermediate meta stable energy level which accepts resonance energy from the sensitiser excited state level.

Abstract: A system, device and/or method for multiplex assays. In a particular, but non-limiting, example there is provided a multiplex array, such as a suspension array. Luminescence decay lifetimes are utilised for probes in a suspension array, and coding/decoding the codes from time-resolved spectra. Lifetime populations can be generated at distinct colour bands. A novel temporal technique or dimension is applied over conventional spectral and intensity combinations, thereby expanding the multiplexing capacity of a suspension array. In one example form, the multiplexing capacity of a suspension array can be expanded to the order of about 58. This provides a reliable, high-throughput and relatively inexpensive solution for multiplex assays in various areas of application such as life sciences, data storage and security.

Abstract: In one form, a two-directional scanning method for luminescence microscopy is disclosed. A series of continuous scans are performed by an interrogation wide-field relative to a first direction and a target is identified. A precise position of the target is determined in the first direction. At least one scan by the interrogation wide-field is performed relative to a second direction at or near the precise position of the target in the first direction. The two-directional scanning method produces “on-the-fly” (i.e. ex tempore or impromptu) precise localization of targets. Embodiments open up new applications for background-free or background-reduced luminescence microscopy, for example time-gated or time-resolved luminescence microscopy, in a relatively fast, higher speed or more efficient manner.