Abstract: Provided is a method for biosynthesis of (R)-?-hydroxytetradecanoate compounds. The method comprises the following steps of: reacting ?-carbonyl tetradecanoate in a ketoreductase and a glucose dehydrogenase or a fusion enzyme thereof, glucose, NADP+ and a buffer solution to obtain (R)-?-hydroxytetradecanoate compounds. The method is an enzyme-catalyzed biosynthesis method having simple operations, conventional equipment and an environmentally friendly process, and the prepared product has high purity, high yield and a high ee value.

Abstract: An electric cylinder driving device includes an electric cylinder outer tube, an electric cylinder inner tube, a ball screw, an electric cylinder piston, a ball screw nut and an elastic oil scraping device. The electric cylinder outer tube is sleeved on the electric cylinder inner tube. The ball screw nut is installed in an upper end of the electric cylinder inner tube. An end of the ball screw is movably connected in the electric cylinder outer tube, and another end of the ball screw matched with the ball screw nut is inserted into the electric cylinder inner tube. The electric cylinder piston is disposed between the electric cylinder outer tube and the electric cylinder inner tube. A lower end of the electric cylinder inner tube is provided with an oil pool. The elastic oil scraping device is disposed between the electric cylinder piston and the electric cylinder outer tube.

Abstract: The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.

Abstract: The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: an upstream fusion primer designed according to the target amplicon, a downstream fusion primer designed according to the target amplicon, an upstream universal primer and a downstream universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.