Patents by Inventor Deb Chatterjee

Deb Chatterjee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190135869
    Abstract: The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
    Type: Application
    Filed: July 20, 2018
    Publication date: May 9, 2019
    Inventors: Deb Chatterjee, Stanislaw Jan Kaczmarczyk
  • Patent number: 10040830
    Abstract: The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: August 7, 2018
    Assignee: The United States of America, as represented by the Secretary, Dept. of Health and Human Services
    Inventors: Deb Chatterjee, Stanislaw Jan Kaczmarczyk
  • Patent number: 9409942
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: August 9, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder
  • Publication number: 20160199511
    Abstract: The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
    Type: Application
    Filed: March 28, 2016
    Publication date: July 14, 2016
    Inventors: Deb Chatterjee, Stanislaw Jan Kaczmarczyk
  • Patent number: 9296790
    Abstract: The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
    Type: Grant
    Filed: October 2, 2009
    Date of Patent: March 29, 2016
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Deb Chatterjee, Stanislaw Jan Kaczmarcyk
  • Publication number: 20160024494
    Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention.
    Type: Application
    Filed: October 5, 2015
    Publication date: January 28, 2016
    Inventors: Mekbib ASTATKE, Deb Chatterjee, Gary Gerard
  • Patent number: 8519099
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: August 27, 2013
    Assignee: Life Technologies Corporation
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder
  • Publication number: 20110189159
    Abstract: The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
    Type: Application
    Filed: October 2, 2009
    Publication date: August 4, 2011
    Applicant: The United states of America as presented by the S deparetment of Health and human services
    Inventors: Deb Chatterjee, Stanislaw Jan Kaczmarcyk
  • Publication number: 20070269878
    Abstract: The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides.
    Type: Application
    Filed: March 23, 2007
    Publication date: November 22, 2007
    Applicant: INVITROGEN CORPORATION
    Inventors: Gary Gerard, Michael Smith, Deb Chatterjee
  • Publication number: 20070190606
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Application
    Filed: December 29, 2006
    Publication date: August 16, 2007
    Applicant: INVITROGEN CORPORATION
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder
  • Publication number: 20070184527
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Application
    Filed: December 29, 2006
    Publication date: August 9, 2007
    Applicant: INVITROGEN CORPORATION
    Inventors: Deb CHATTERJEE, Mary LONGO, Elizabeth FLYNN, Robert OBERFELDER
  • Publication number: 20070178489
    Abstract: The present invention relates to polypeptides, compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses polypeptides having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis and more particularly to polymerases having reduced polymerase and optionally reduced exonuclease activities (3? to 5? and/or 5? to 3? exonuclease activity), and to nucleases having reduced nuclease activity. The polypeptides of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature.
    Type: Application
    Filed: October 6, 2006
    Publication date: August 2, 2007
    Applicant: Invitrogen Corporation
    Inventors: Mekbib Astatke, Deb Chatterjee, Harini Shadilya
  • Publication number: 20070087356
    Abstract: The invention provides a microarray and methods for producing a protein microarray. The array comprises multiple nucleic acid molecules immobilized on a substrate, each comprising (i) a protein-binding domain and (ii) a nucleic acid sequence encoding a fusion protein comprising a polypeptide of interest and a DNA-binding protein that binds the protein-binding domain, and one or more fusion proteins produced from the multiple nucleic acid molecules. Each fusion protein is immobilized on the substrate via binding to a nucleic acid sequence comprising the protein-binding domain present on the nucleic acid molecule from which the fusion protein is produced or on the substrate. The invention also provides a method of analyzing protein interactions with, for example, other proteins, lipids and drugs.
    Type: Application
    Filed: October 19, 2005
    Publication date: April 19, 2007
    Applicant: Government of the USA, represented by the Secretary, Department of Health & Human Services
    Inventors: Deb Chatterjee, Kalavathy Sitaraman, James Hartley, Cassio Baptista, David Munroe
  • Publication number: 20060286633
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Application
    Filed: January 11, 2006
    Publication date: December 21, 2006
    Applicant: Invitrogen Corporation
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder
  • Publication number: 20060269992
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Application
    Filed: January 11, 2006
    Publication date: November 30, 2006
    Applicant: Invitrogen Corporation
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder
  • Publication number: 20060134739
    Abstract: In Vitro peptide/protein or biological macromolecule synthesis systems, and methods and kits thereof improve efficiency of in vitro protein or biological macromolecule synthesis and related compositions.
    Type: Application
    Filed: March 10, 2004
    Publication date: June 22, 2006
    Inventor: Deb Chatterjee
  • Publication number: 20050123905
    Abstract: The present invention is directed generally to methods facilitating the cloning of nucleic acid molecules. In particular, the invention relates to the use of polymerase inhibitors, including but not limited to anti-polymerase antibodies (such as anti-Taq antibodies) and fragments thereof, to inactivate residual polymerase activity remaining after the amplification (particularly via PCR) of a target nucleic acid molecule. The invention further provides compositions, particularly storage-stable compositions, comprising one or more components, such as one or more restriction endonucleases and one or more polymerase inhibitors, that are useful in cloning amplified or synthesized nucleic acid molecules by the above-described methods. The invention also relates to nucleic acid molecules produced by these methods, and to genetic constructs (such as vectors) and host cells comprising these nucleic acid molecules.
    Type: Application
    Filed: October 8, 2004
    Publication date: June 9, 2005
    Applicant: Invitrogen Corporation
    Inventors: Donna Fox, Deb Chatterjee
  • Publication number: 20050112637
    Abstract: The present invention relates to mutant DNA polymerases which incorporate dideoxynucleotides with about the same efficiency as deoxynucleotides. The present invention also related to mutant DNA polymerases which also have substantially reduced 5?-to-3? exonuclease activity or 3?-to-5? exonuclease activity. The invention also relates to DNA molecules coding for the mutant DNA polymerases, and hosts containing the DNA molecules.
    Type: Application
    Filed: September 23, 2004
    Publication date: May 26, 2005
    Inventor: Deb Chatterjee
  • Publication number: 20050089922
    Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention.
    Type: Application
    Filed: December 9, 2004
    Publication date: April 28, 2005
    Inventors: Mekbib Astatke, Deb Chatterjee, Gary Gerard
  • Patent number: 6703484
    Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.
    Type: Grant
    Filed: January 8, 1998
    Date of Patent: March 9, 2004
    Assignee: Invitrogen Corporation
    Inventors: Deb Chatterjee, Mary Longo, Elizabeth Flynn, Robert Oberfelder