Abstract: A tissue culture method and a propagation method of Cathaya argyrophylla are described herein. An adventitious bud can be induced from an explant of the Cathaya argyrophylla using a suitable induction medium, with an induction rate reaching 69.22% to 73.33%. The induced adventitious bud is inoculated into a suitable proliferation medium, such that the adventitious bud can proliferate and strengthen seedlings, where the adventitious bud shows a proliferation rate reaching 46.23% to 55%. The adventitious bud after proliferation and seedling strengthening are inoculated into a suitable rooting medium to allow rooting culture, thereby obtaining a complete tissue culture seedling of the Cathaya argyrophylla. The tissue culture method is of great significance for protecting the Cathaya argyrophylla, increasing a plant quantity of the Cathaya argyrophylla, and expanding a population of the Cathaya argyrophylla to prevent extinction.