Abstract: A garment is provided that includes an outer tubular garment portion and an inner tubular garment portion that are each fabricated from the same, continuous piece of protective material. By being formed from continuous portion of material, a user is provided with protection from ingress of fluids around an appendage when such fluid might be otherwise introduced through breaks in the material that rely entirely on a circumferential seam to secure separate pieces of material together. The inner tubular portion is not removable or severable from the outer tubular garment portion but is extendible to allow a user flexibility of use (e.g., stretching) without sacrificing performance. A method of creating a circumferentially continuous fluid impervious seal is also provided.

Abstract: A mycobacterium attenuated by a mutation in a gene such as the proC or trpD that expresses a product which promotes synthesis of proline or tryptophan is used in a method of vaccinating a host. The mycobacterium may be Mycobacterium tuberculosis or Mycobacterium bovis.