Patents by Inventor Debkishore Mitra

Debkishore Mitra has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190309356
    Abstract: Colorimetry is used to detect amplification reaction products. A sample is contacted with a reaction mix under conditions such that an amplification reaction occurs and produces an amplification reaction product if the sample contains a target nucleic acid template molecule. The reaction mix includes an enzyme for catalyzing the amplification reaction, and at least one halochromic agent. If the target nucleic acid template molecule is present, the amplification reaction changes the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating the presence of the target nucleic acid. If the target nucleic acid template molecule is not present, the amplification reaction does not generate an adequate number of protons to sufficiently change the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating that the amplification reaction product has not been produced.
    Type: Application
    Filed: March 20, 2019
    Publication date: October 10, 2019
    Inventors: Debkishore Mitra, Ivan Krastev Dimov, John Robert Waldeisen
  • Patent number: 10343166
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: July 9, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Patent number: 10253357
    Abstract: Colorimetry is used to detect amplification reaction products. A sample is contacted with a reaction mix under conditions such that an amplification reaction occurs and produces an amplification reaction product if the sample contains a target nucleic acid template molecule. The reaction mix includes an enzyme for catalyzing the amplification reaction, and at least one halochromic agent. If the target nucleic acid template molecule is present, the amplification reaction changes the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating the presence of the target nucleic acid. If the target nucleic acid template molecule is not present, the amplification reaction does not generate an adequate number of protons to sufficiently change the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating that the amplification reaction product has not been produced.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: April 9, 2019
    Assignee: DIASSESS INC.
    Inventors: Debkishore Mitra, Ivan Krastev Dimov, John Robert Waldeisen
  • Publication number: 20190094114
    Abstract: Devices and methods for preparing and delivering biological assay samples are provided herein. Components of such devices include a sample receiving module within which a biological assay sample can be prepared and a cap, which when operatively coupled with the sample receiving module, pressurizes the module. These devices can be employed for subsequently delivering a biological assay sample.
    Type: Application
    Filed: March 14, 2017
    Publication date: March 28, 2019
    Inventors: Frank B. Myers, III, Wei Hsuan Ho, Debkishore Mitra, John Robert Waldeisen, Ivan Krastev Dimov, Ryan C. Griswold, Bruce Richardson
  • Publication number: 20190083975
    Abstract: Systems and methods for performing biological assays are provided herein. The systems and methods determine one or more characteristics of a nucleic acid amplification sample based on a modified optical property of the sample.
    Type: Application
    Filed: March 14, 2017
    Publication date: March 21, 2019
    Inventors: Debkishore Mitra, Frank B. Myers, III, John Robert Waldeisen, Ivan Krastev Dimov
  • Publication number: 20190060895
    Abstract: Devices and methods for modifying optical properties of biological samples or aspects thereof are provided. The subject methods include generating a reaction product with a device and reacting the reaction product to sufficiently modify an optical property to allow detection of the modified optical property.
    Type: Application
    Filed: March 14, 2017
    Publication date: February 28, 2019
    Inventors: Frank B. Myers, III, Debkishore Mitra, John Robert Waldeisen, Ivan Krastev Dimov
  • Publication number: 20190050988
    Abstract: A diagnostic system performs disease diagnostic tests using at least an optical property modifying device and a mobile device. A user provides a biological sample from a patient to the optical property modifying device that reacts with a reagent in one or more reaction chambers of the device. The user captures one or more images of the one or more reaction chambers using an optical sensor of the mobile device. The diagnostic system can determine a quality level of the images based on factors such as skew, scale, focusing, shadowing, or white-balancing. Based on an analysis of the captured image, the diagnostic system can determine a test result of a disease diagnostic test for the patient. The diagnostic system may communicate the test result, as well as instructions for the disease diagnostic test, to the user via the mobile device.
    Type: Application
    Filed: October 9, 2018
    Publication date: February 14, 2019
    Inventors: Ivan Krastev Dimov, Frank B. Myers, III, John Robert Waldeisen, Debkishore Mitra
  • Patent number: 10150117
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Patent number: 10146909
    Abstract: A diagnostic system performs disease diagnostic tests using at least an optical property modifying device and a mobile device. A user provides a biological sample from a patient to the optical property modifying device that reacts with a reagent in one or more reaction chambers of the device. The user captures one or more images of the one or more reaction chambers using an optical sensor of the mobile device. The diagnostic system can determine a quality level of the images based on factors such as skew, scale, focusing, shadowing, or white-balancing. Based on an analysis of the captured image, the diagnostic system can determine a test result of a disease diagnostic test for the patient. The diagnostic system may communicate the test result, as well as instructions for the disease diagnostic test, to the user via the mobile device.
    Type: Grant
    Filed: April 6, 2017
    Date of Patent: December 4, 2018
    Assignee: Diassess Inc.
    Inventors: Ivan Krastev Dimov, Frank B. Myers, III, John Robert Waldeisen, Debkishore Mitra
  • Patent number: 10137449
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: November 27, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20180293350
    Abstract: A diagnostic system performs disease diagnostic tests using at least an optical property modifying device and a mobile device. A user provides a biological sample from a patient to the optical property modifying device that reacts with a reagent in one or more reaction chambers of the device. The user captures one or more images of the one or more reaction chambers using an optical sensor of the mobile device. The diagnostic system can determine a quality level of the images based on factors such as skew, scale, focusing, shadowing, or white-balancing. Based on an analysis of the captured image, the diagnostic system can determine a test result of a disease diagnostic test for the patient. The diagnostic system may communicate the test result, as well as instructions for the disease diagnostic test, to the user via the mobile device.
    Type: Application
    Filed: April 6, 2017
    Publication date: October 11, 2018
    Inventors: Ivan Krastev Dimov, Frank B. Myers, III, John Robert Waldeisen, Debkishore Mitra
  • Patent number: 10071377
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyzes and characterization.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: September 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20180015472
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Application
    Filed: September 27, 2017
    Publication date: January 18, 2018
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20180015473
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Application
    Filed: September 27, 2017
    Publication date: January 18, 2018
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20180008984
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Application
    Filed: August 28, 2017
    Publication date: January 11, 2018
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20170348691
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Application
    Filed: May 16, 2017
    Publication date: December 7, 2017
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Patent number: 9737888
    Abstract: An apparatus with a self-contained, tunable, microfluidic pumping system that utilizes the high air permeability of the matrix material to actuate fluid flow in a network of fluidic microchannels and microstructures is provided. The pumping relies upon partial evacuation of degas/vacuum channels that are located next to the fluid channels to degas air from the fluid channels or structures producing a reduction of pressure in the fluidic channel leading to the flow of fluid from an inlet at atmospheric pressure through the device. The solution is isolated from the pumping apparatus since the liquid does not pass through the diffusion barriers. The apparatus and method can also provide bubble-free microfluidic pumping, without any auxiliary equipment or device pre-treatment, and can fill dead-end channels and chambers, providing a powerful liquid handling tool for a broad range of applications.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: August 22, 2017
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Luke P. Lee, Debkishore Mitra, John R. Waldeisen, Jixiao Liu
  • Patent number: 9694361
    Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: July 4, 2017
    Assignee: 10X Genomics, Inc.
    Inventors: Rajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
  • Publication number: 20170044599
    Abstract: Colorimetry is used to detect amplification reaction products. A sample is contacted with a reaction mix under conditions such that an amplification reaction occurs and produces an amplification reaction product if the sample contains a target nucleic acid template molecule. The reaction mix includes an enzyme for catalyzing the amplification reaction, and at least one halochromic agent. If the target nucleic acid template molecule is present, the amplification reaction changes the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating the presence of the target nucleic acid. If the target nucleic acid template molecule is not present, the amplification reaction does not generate an adequate number of protons to sufficiently change the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating that the amplification reaction product has not been produced.
    Type: Application
    Filed: April 24, 2015
    Publication date: February 16, 2017
    Inventors: Debkishore Mitra, Ivan Krastev Dimov, John Robert Waldeisen
  • Publication number: 20160144362
    Abstract: An apparatus with a self-contained, tunable, microfluidic pumping system that utilizes the high air permeability of the matrix material to actuate fluid flow in a network of fluidic microchannels and microstructures is provided. The pumping relies upon partial evacuation of degas/vacuum channels that are located next to the fluid channels to degas air from the fluid channels or structures producing a reduction of pressure in the fluidic channel leading to the flow of fluid from an inlet at atmospheric pressure through the device. The solution is isolated from the pumping apparatus since the liquid does not pass through the diffusion barriers. The apparatus and method can also provide bubble-free microfluidic pumping, without any auxiliary equipment or device pre-treatment, and can fill dead-end channels and chambers, providing a powerful liquid handling tool for a broad range of applications.
    Type: Application
    Filed: November 20, 2015
    Publication date: May 26, 2016
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Luke P. Lee, Debkishore Mitra, John R. Waldeisen, Jixiao Liu