Patents by Inventor Deborah Boles
Deborah Boles has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9840737Abstract: The present invention relates to methods and systems for genome scanning using high resolution melting analysis for identifying mutations and/or variants in genes of interest.Type: GrantFiled: June 2, 2011Date of Patent: December 12, 2017Assignee: Canon U.S. Life Sciences, Inc.Inventors: Jacquline Yancy, Lingxia Jiang, Stephanie Buszczak, Renee Howell, Deborah Boles
-
Publication number: 20160342736Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. Methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: ApplicationFiled: March 21, 2016Publication date: November 24, 2016Applicant: Canon U.S. Life Sciences, Inc.Inventors: Deborah BOLES, Kenton C. HASSON, Sami KANDERIAN
-
Patent number: 9292653Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: GrantFiled: December 10, 2013Date of Patent: March 22, 2016Assignee: Canon U.S. Life Sciences, Inc.Inventors: Deborah Boles, Kenton C. Hasson, Sami Kanderian
-
Publication number: 20150065360Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.Type: ApplicationFiled: November 11, 2014Publication date: March 5, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Allen E. Eckhardt, Jonathan Benton, Deborah Boles
-
Patent number: 8901043Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.Type: GrantFiled: July 6, 2012Date of Patent: December 2, 2014Assignee: Advanced Liquid Logic, Inc.Inventors: Allen E. Eckhardt, Jonathan Benton, Deborah Boles
-
Publication number: 20140180600Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: ApplicationFiled: December 10, 2013Publication date: June 26, 2014Applicant: Canon U.S. Life Sciences, Inc.Inventors: Deborah BOLES, Kenton C. HASSON, Sami KANDERIAN
-
Publication number: 20140005066Abstract: The present invention provides a droplet actuator device and methods for multiplexed PCR amplification and detection of target amplicons within a single droplet. The methods of the invention combine quantitative real-time PCR (qPCR) amplification with fluorescence-based sequence specific detection technologies for amplified DNA. In one embodiment, fluorescently-labeled oligonucleotide probes may be used for hybridization-based multiplexed detection of target amplicons. The methods of the invention generally involve combining the necessary reactants to form a PCR-ready droplet and thermal cycling the droplet at temperatures sufficient to result in amplification of one or more target nucleic acids. Fluorescence-based detection techniques may be used for end-point or real-time analysis of DNA amplification. For end-point analysis, the accumulation of a signal, e.g., a fluorescence signal, is measured after the amplification of the target sequence is complete.Type: ApplicationFiled: July 1, 2013Publication date: January 2, 2014Applicant: ADVANCED LIQUID LOGIC INC.Inventors: Deborah Boles, Lisa Perkins, Allen E. Eckhardt, Jennifer Foley
-
Patent number: 8606529Abstract: The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: GrantFiled: March 26, 2012Date of Patent: December 10, 2013Assignee: Canon U.S. Life Sciences, Inc.Inventors: Deborah Boles, Kenton C. Hasson, Sami Kanderian
-
Publication number: 20130178374Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.Type: ApplicationFiled: July 6, 2012Publication date: July 11, 2013Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Allen E. Eckhardt, Jonathan Benton, Deborah Boles
-
Publication number: 20120239305Abstract: The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: ApplicationFiled: March 26, 2012Publication date: September 20, 2012Applicant: Canon U.S. Life Sciences, Inc.Inventors: Deborah BOLES, Kenton C. Hasson, Sami Kanderian
-
Publication number: 20120088236Abstract: The present invention relates to methods and systems for genome scanning using high resolution melting analysis for identifying mutations and/or variants in genes of interest.Type: ApplicationFiled: June 2, 2011Publication date: April 12, 2012Applicant: CANON U.S. LIFE SCIENCES, INC.Inventors: Jacquline Yancy, Lingxia Jiang, Stephanie Buszczak, Renee Howell, Deborah Boles
-
Patent number: 8145433Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: GrantFiled: October 24, 2008Date of Patent: March 27, 2012Assignee: Canon U.S. Life Sciences, Inc.Inventors: Deborah Boles, Kenton C. Hasson, Sami Kanderian
-
Publication number: 20090112484Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.Type: ApplicationFiled: October 24, 2008Publication date: April 30, 2009Applicant: CANON U.S. LIFE SCIENCES, INC.Inventors: Deborah BOLES, Kenton C. HASSON, Sami KANDERIAN
-
Publication number: 20070026421Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: ApplicationFiled: February 9, 2006Publication date: February 1, 2007Applicants: Caliper Life Sciences, Inc., Canon U.S. Life Sciences, Inc.Inventors: Steven Sundberg, Michael Knapp, Ivor Knight, Deborah Boles, Aaron Rulison, Wesley Dong, Edward Donlon, Robert Moti, Andrew Fabans, Allen Boronkay, Michael Slater
-
Publication number: 20060115830Abstract: The invention provides novel SGP primers for improved use in waveform-profiling methods of DNA amplification. In one embodiment, use of an SGP primer in a method of DNA amplification results in exponential amplification of several distinct products. In another embodiment, the methods of the invention further comprise a novel half-time elongation step. In another embodiment, the distinct products may be detected via melting temperature analysis. The primers and methods of the invention may be combined to determine an organism in a sample.Type: ApplicationFiled: July 28, 2005Publication date: June 1, 2006Inventors: Toru Takahashi, Deborah Boles