Patents by Inventor Deborah Polayes

Deborah Polayes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    Patent number: 7872116
    Abstract: The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.
    Type: Grant
    Filed: February 6, 2007
    Date of Patent: January 18, 2011
    Assignee: American Type Culture Collection (ATCC)
    Inventors: Pranvera Ikonomi, Deborah Polayes, Karin Cottrill, Quanyi Li
  • Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    Publication number: 20080187916
    Abstract: The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.
    Type: Application
    Filed: February 6, 2007
    Publication date: August 7, 2008
    Inventors: Pranvera Ikonomi, Deborah Polayes, Karin Cottrill, Quanyi Li