Abstract: The invention relates to a method for decellularization of tissue samples to produce decellularized extracellular matrix (dECM) by performing one or more wash cycles in a reactor using a reagent after at least partially submerging the tissue sample in the reagent, wherein the reactor comprising a tissue chamber in fluid communication with a reactor chamber. The method further comprising the steps of agitating the tissue sample and/or the reagent such that the tissue sample circulates in the reagent while retaining the tissue sample in a tissue chamber or a decellularization reactor, monitoring at least one parameter of the reagent and/or at least one parameter of the tissue sample, and adjusting one or more wash cycles based on said monitoring. The invention further relates to a decellularization reactor and a decellularization system for decellularizing a tissue sample.

Abstract: A method of culturing cells or tissues is provided. The method comprises a) providing a support, wherein the support is an optical medium with a patterned surface; b) contacting the support with cells and a culture medium; and c) culturing the cells under suitable cultivation conditions. A device for cell or tissue culture comprising an optical medium, wherein a patterned surface of the optical medium forms a support upon which cells are cultivated, is also provided.

Abstract: A method of monitoring activity of an animal, the method comprising the steps of: retaining the animal in an enclosed chamber; controlling a food supply to the animal in the enclosed chamber as a first stimulus; and monitoring the activity of the animal in response to the first stimulus, wherein monitoring the activity of the animal comprises analysing change in a volume of food in the food supply and location of the animal in the enclosed chamber over a predetermined period of time.