Abstract: The invention relates to compositions and methods for making and use of a real-time cellular sensor. Components of a multipart enzyme are sequestered in different cellular compartments and only come together after receptor activation; a pool of substrate is made available in the cell to ensure real-time enzymatic output.

Abstract: A tissue-engagement member having a pair of jaws movable between a closed configuration and an open configuration, an actuator movable to shift the jaws between the closed and open configurations, and a biasing element biasing the jaws into a closed configuration. The biasing element may be provided over a portion of the tissue-engagement member. A cam mechanism may be provided in operative association with at least one of the jaws and/or the actuator. The biasing element may operatively engage at least one of the jaws and/or the actuator to actuate the cam mechanism to return the jaws to a closed configuration from an open configuration. A manipulator having an actuator-engagement element may be provided to engage an actuation element operatively associated with the actuator. The actuator-engagement element may operatively engage the actuation element in a range of orientations.

Abstract: Provided herein are sequestrons for detecting an miRNA profile indicative of a cell state and expressing an output molecule in cells having such an miRNA profile. Also provided are methods of using sequestrons provided herein.

Abstract: A method for forming an acrylic emulsion includes a step of forming pre-emulsion by combining a monomer composition with an amphoteric surfactant in water and then polymerizing the pre-emulsion by combining the pre-emulsion with a radical initiator in a reactor to form a reaction mixture. The monomer composition includes one or more monomers selected from the group consisting of (meth)acrylic acid monomers and (meth)acrylic monomers, and combinations thereof. An acrylic emulsion formed by the method is also provided.