Abstract: Provided is a method for producing 1,3-propanediol by means of fermentation of a recombinant microorganism. First, a recombinant microorganism is provided; the recombinant microorganism can overexpress acetyl-CoA carboxylase genes: accBC and accDA, a malonyl-CoA synthetase gene: mcr, a 3-hydroxypropionyl-CoA synthetase gene: pcs, a 3-hydroxypropionyl-CoA reductase gene: pduP, and a 1,3-propanediol reductase gene: yqhD. The recombinant microorganism is subjected to fermentation culture in a flask or fermentor using glucose ad as raw material to obtain the 1,3-propanediol. The recombinant microorganism can utilize low-cost glucose, sucrose, molasses, xylose and the like as raw material in the fermentation process, without additional expensive vitamin B12. Thus, cost of the production is significantly reduced, and there is a promising prospect in market.

Abstract: The invention provides a method for preparing drinking weak alkali water and strontium-rich electrolyte raw water from distilled water obtained in the process of producing salt from well and mine brine, which comprises procedures of pressure reduction treatment, primary filtration, membrane separation treatment, activated carbon adsorption treatment, secondary filtration treatment, sterilization and disinfection treatment and the like on the salt-making distilled water in sequence so as to remove insoluble solid impurities, sulfides, microorganisms and other substances in the raw water, and separate or enrich ions, thereby preparing the weak alkali water suitable for drinking and the strontium-rich electrolyte raw water capable of being used for functional beverage production. The method provided by the invention can not only realize the comprehensive utilization of resources, but also obtain new products, significantly improving the economical efficiency of the process.

Abstract: A method for producing ectoine by fermenting recombinant Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum is obtained by overexpressing, in Corynebacterium glutamicum, an aspartokinase gene lysC of which feedback inhibition is relieved, then replacing the promoter of the dihydrodipicolinate synthase in the recombinant bacterium to attenuate the activity of the dihydropyrimidine dicarboxylic acid synthase, and then transforming the recombinant bacterium with the ectoine synthetic path related gene ectABC. The recombinant Corynebacterium glutamicum can be fermented using different cheap raw materials under a low salt condition to produce ectoine, and use cheap corn slurry instead of expensive yeast powder as a nutritional component, so as to further reduce the costs of the raw materials. In addition, the recombinant Corynebacterium glutamicum solves the biosafety problem, simplifies the post-extraction process, and has a good market application prospect.

Abstract: Provided is a method for treating diseases related to cardiac tissue damage or cardiac insufficiency in a subject. The method includes the step of locally applying a mesenchymal stem cell sheet such as an umbilical cord mesenchymal stem cell sheet to the heart of the subject. Also provided are related use and compositions of the mesenchymal stem cell sheet.

Abstract: Provided are a cleaning machine, a cleaning device, an information display method thereof, and a storage medium. A display is additionally arranged on a main body of the cleaning machine to display working state information of at least one component on the cleaning machine, thereby visually displaying a working state of the cleaning machine. A user may know about a working state of a component on the cleaning machine visually, which contributes to improving user experiences.

Abstract: A process for producing para-xylene and ethylbenzene from C8 aromatics containing ethylbenzene includes the steps of sending C8 aromatics containing ethylbenzene to an ethylbenzene liquid-phase adsorption separation device, wherein a suction liquid containing ethylbenzene and a suction residual liquid are obtained after the adsorption separation, and the desorbents in the suction liquid and the suction residual liquid are removed to obtain ethylbenzene and a suction residual oil; sending the suction residual oil to a para-xylene adsorption separation device, and unadsorbed components are discharged as a raffinate from the adsorbent bed; the adsorbent bed is rinsed with a desorbent to desorb the para-xylene therein and obtain an extract; the desorbents in the extract and the raffinate are respectively removed to obtain para-xylene and a raffinate oil; sending the raffinate oil to a xylene isomerization device to carry out xylene isomerization, and the isomerization product is fractionated.

Abstract: Systems and methods providing optical structures providing a large field of view (FOV) for personal portable displays are described. An optical structure of embodiments comprises a catadioptric optical configuration configured to produce a magnified curved intermediate image, wherein the magnified curved intermediate image is furthered magnified with proper reflection angle and transmitted to viewer's eye. The catadioptric optical configuration may comprise two curved mirrors and a polarization prism. Optical structures comprising such catadioptric optical configurations may utilize relatively small sized image sources to produce a large FOV. Such optical structures are well suited for use in large FOV optical see through (OST) head-mounted displays (HMDs).

Abstract: A method for cryopreserving cells, a cell cryopreservation solution and a composition are provided. The method includes: providing a suspension for the cells to be cryopreserved in a cell cryopreservation solution; and cryopreserving the suspension. The cell cryopreservation solution includes dimethyl sulfoxide, plasma, citric acid, sodium citrate, potassium dihydrogen phosphate or sodium dihydrogen phosphate, glucose and adenine.

Abstract: An artificial intelligence cloud diagnosis platform is provided. A subject sample is scanned to a cloud side, AI on the cloud conducts preliminary detection, and then a doctor carries out diagnosis and rechecking. The artificial intelligence cloud diagnosis improves the diagnosis accuracy, fundamentally solves the problems of insufficient technical experts and non-uniform medical resource distribution in remote areas, greatly improves the diagnosis efficiency, reduces the diagnosis cost, establishes a professional database of the same type of detection samples, and is beneficial to the further research of a disease in the later period.

Abstract: Disclosed is an NADH-dependent amino acid dehydrogenase and an application thereof in increasing lysine yield. The amino acid dehydrogenases are aspartate dehydrogenase derived from Pseudomonas aeruginos, aspartate semialdehyde dehydrogenase derived from Tistrella mobilis, dihydropyridine dicarboxylic acid reductase derived from Mycobacterium tuberculosis, and diaminopimelate dehydrogenase derived from Tepidanaerobacter acetatoxydans. The amino acid sequences thereof are as shown in SEQ ID NOs: 1, 3, 5, and 7, respectively. NADH or both NADH and NADPH can be used as co-factors of the amino acid dehydrogenase to synthesize lysine, thereby reducing the demand for NADPH in the cell, and significantly increasing the production of lysine or pentanediamine.

Abstract: Provided in the present invention is a method for improving the yield of enzymatic preparation of biodiesel from greases, comprising: during enzymatic reaction of short-chain alcohols with greases to prepare biodiesel, introducing online dehydration steps, which lead the volatile gases in the enzyme reactor to enter into the low-temperature absorption tank, and the dehydrated gases flow back into the enzyme reactor. The formation of gas-circulation can constantly bring the moisture inside the enzyme reactor out.

Abstract: Provided is a method for producing 1,3-propanediol by means of fermentation of a recombinant microorganism. First, a recombinant microorganism is provided; the recombinant microorganism can overexpress acetyl-CoA carboxylase genes: accBC and accDA, a malonyl-CoA synthetase gene, mcr, a 3-hydroxypropionyl-CoA synthetase gene: pcs, a 3-hydroxypropionyl-CoA reductase gene: pduP, and a 1,3-propanediol reductase gene: yqhD. The recombinant microorganism is subjected to fermentation culture in a flask or ferment or using glucose ad as raw material to obtain the 1,3-propanediol. The recombinant microorganism can utilize low-cost glucose, sucrose, malasses, xylose and the like as raw material in the fermentation process, without additional expensive vitamin B12. Thus, cost of the production is significantly reduced, and there is a promising prospect in market.

Abstract: The invention provides a method for preparing drinking weak alkali water and strontium-rich electrolyte raw water from distilled water obtained in the process of producing salt from well and mine brine, which comprises procedures of pressure reduction treatment, primary filtration, membrane separation treatment, activated carbon adsorption treatment, secondary filtration treatment, sterilization and disinfection treatment and the like on the salt-making distilled water in sequence so as to remove insoluble solid impurities, sulfides, microorganisms and other substances in the raw water, and separate or enrich ions, thereby preparing the weak alkali water suitable for drinking and the strontium-rich electrolyte raw water capable of being used for functional beverage production. The method provided by the invention can not only realize the comprehensive utilization of resources, but also obtain new products, significantly improving the economical efficiency of the process.

Abstract: The invention provides a method for preparing strontium-rich mineral water from distilled water obtained in the salt production process from brine, which comprises the following steps: carrying out aeration pretreatment on the salt-making distilled water to obtain pretreated distilled water, and further performing sand filtration, ion exchange, activated carbon, membrane filtration and sterilization and disinfection treatment on the pretreated distilled water to obtain the drinking strontium-rich mineral water with the strontium element content of more than 0.4 mg/L.

Abstract: Systems and methods providing optical structures providing a large field of view (FOV) for personal portable displays are described. An optical structure of embodiments comprises a catadioptric optical configuration configured to produce a magnified curved intermediate image, wherein the magnified curved intermediate image is furthered magnified with proper reflection angle and transmitted to viewer's eye. The catadioptric optical configuration may comprise two curved mirrors and a polarization prism. Optical structures comprising such catadioptric optical configurations may utilize relatively small sized image sources to produce a large FOV. Such optical structures are well suited for use in large FOV optical see through (OST) head-mounted displays (HMDs).

Abstract: A method for producing ectoine by fermenting recombinant Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum is obtained by overexpressing, in Corynebacterium glutamicum, an aspartokinase gene lysC of which feedback inhibition is relieved, then replacing the promoter of the dihydrodipicolinate synthase in the recombinant bacterium to attenuate the activity of the dihydropyrimidine dicarboxylic acid synthase, and then transforming the recombinant bacterium with the ectoine synthetic path related gene ectABC. The recombinant Corynebacterium glutamicum can be fermented using different cheap raw materials under a low salt condition to produce ectoine, and use cheap corn slurry instead of expensive yeast powder as a nutritional component, so as to further reduce the costs of the raw materials. In addition, the recombinant Corynebacterium glutamicum solves the biosafety problem, simplifies the post-extraction process, and has a good market application prospect.

Abstract: The present invention provides table salts. The said table salts contains 50 to 600 mg/kg of strontium. The strontium-rich table salts provided by the present invention has significantly higher strontium content than traditional common table salts. As one of the essential trace elements, strontium plays an important role in human health. The said table salts provided by the present invention are rich in strontium, which can be assimilated by human body during taking the said table salts. Moreover, the strontium content in the table salts provided by the present invention ranges from 50 to 600 mg/kg, which enables human body to get enough strontium to meet human needs when taking normal amount of the said table salts daily.

Abstract: The present invention provides a method for preparing biodiesel, comprising: adding oil and fat, short-chain alcohol, water, and liquid lipase into a single-stage or multi-stage enzyme reactor; then separating the reaction fluid into an enzyme-containing heavy phase and a light phase; recovering and reusing the enzyme in the heavy phase; using the light phase for subsequent conversion by immobilized lipase; flowing the light phase and the short-chain alcohol into single-stage or multi-stage enzyme reactor containing an immobilized lipase; performing online dehydration during the whole reaction process or part of the reaction process.

Abstract: Disclosed is a process for preparing biodiesel from renewable oil and fat catalyzed by lipase with online dehydration. The method uses a short chain alcohol ROH as an acyl-acceptor, and utilizes a lipase as the catalyst to catalyze transesterification reaction of oil and fat feedstock with said short chain alcohol, so as to synthesize biodiesel; and online dehydration with a membrane or a molecular sieve is carried out during the whole process or part of the reaction process. Such online dehydration process greatly simplifies the operation procedure, and provides good economic and environmental benefits.

Abstract: Provided in the present invention is a method for improving the yield of enzymatic preparation of biodiesel from greases, comprising: during enzymatic reaction of short-chain alcohols with greases to prepare biodiesel, introducing online dehydration steps, which lead the volatile gases in the enzyme reactor to enter into the low-temperature absorption tank, and the dehydrated gases flow back into the enzyme reactor. The formation of gas-circulation can constantly bring the moisture inside the enzyme reactor out.