Abstract: Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.

Abstract: High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.

Abstract: Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.

Abstract: Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.

Abstract: High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.

Abstract: High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.

Abstract: The invention describes a nucleic acid system, named as “2A-transcription amplifier”, for enhancing gene expression by linking a gene of interest (GOI) to a self-amplifying transcription factor with a viral 2A-like peptide. The system comprises an upstream activation sequence (UAS) at upstream promoter region and another sequence encoding a specific transcription factor (TF), a viral 2A-like peptide, and a gene of interest (GOI). The said compositions are operably linked in a way that the initially expressed TF protein binds the UAS region and promotes more TF and GOI co-expression. The viral 2A-like peptide separates the co-expressed TF and GOI protein during protein translation by the mechanism of ribosomal skidding. The system creates a transcription amplification loop that can be employed for enhancing expression of exogenous or endogenous gene of interest (GOI) in eukaryotic cells, tissues or whole organisms.

Abstract: Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.

Abstract: High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.

Abstract: This document provides methods and materials related to transcription terminators. For example, methods and materials related to transcription terminators and nucleic acid molecules (e.g., vectors and constructs) that contain a nucleic acid sequence that encodes a polypeptide operably linked to a transcription terminator are provided. In addition, plants, plant cells, and plant seeds having a nucleic acid sequence that encodes a polypeptide operably linked to a transcription terminator are provided.

Abstract: This document provides methods and materials related to transcription terminators. For example, methods and materials related to transcription terminators and nucleic acid molecules (e.g., vectors and constructs) that contain a nucleic acid sequence that encodes a polypeptide operably linked to a transcription terminator are provided. In addition, plants, plant cells, and plant seeds having a nucleic acid sequence that encodes a polypeptide operably linked to a transcription terminator are provided.