Abstract: The present disclosure discloses a preparation method and application of decellularized extracellular matrix tissue paper, and belongs to the technical field of regenerative medicine. The preparation method includes the following steps: (1) preparing decellularized extracellular matrix materials; (2) preparing tissue paper: homogenizing and stirring the prepared decellularized extracellular matrix materials, then, placing the obtained homogenates on a flat-bottom filter screen to filter out water, standing and air-drying the filtered homogenates, and freeze-drying the homogenates to obtain the tissue paper; (3) cross-linking the tissue paper: cross-linking the tissue paper in a cross-linking solution; and (4) freeze-drying or stacking and compacting the cross-linked tissue paper. According to the present disclosure, the decellularized extracellular matrix tissue paper with tissue specificity is prepared by a traditional paper-making technology combined with a freeze-drying technology.

Abstract: A vascularized cardiac organoid with a chamber structure and its preparation method is presented. The cardiac organoids have a unique chamber structure, which includes a myocardium wall. Within the cardiac organoids, a vascular network is developed, enclosed within the myocardium wall. This vascular network is composed of endothelial cells surrounded by smooth muscle cells, forming a network of branched tubular structures. The presence of vasculature significantly promotes the arrangement of the myocardium, enhances the organization of the cardiac cytoskeleton, and promotes the consistency and beating frequency of cardiac cells. The vasculature facilitates the growth and size increase of the cardiac organoids.

Abstract: The present disclosure discloses a composite tubular material and a preparation method thereof. The tubular material is prepared from extracellular matrix and directionally arranged synthetic polymer fibers through compounding; the synthetic polymer fibers serve as an internal skeleton, a fiber diameter ranges from 1 ?m to 2000 ?m, a fiber angle ranges from 0° to 180°, and a wall thickness ranges from 1 ?m to 1000 ?m; and extracellular matrix components are obtained from human or animal tissue through decellularization. According to the present disclosure, the tubular material with bioactivity and excellent mechanical properties can be prepared from the synthetic polymer and the natural material extracellular matrix through compounding; the synthetic polymer fibers with controllable fiber angle and diameter serve as the internal skeleton of the tubular material, and accordingly the tubular material has the mechanical properties of resistance to bending and squeezing.

Abstract: A vascularized cardiac organoid with a chamber structure and its preparation method is presented. The cardiac organoids have a unique chamber structure, which includes a myocardium wall. Within the cardiac organoids, a vascular network is developed, enclosed within the myocardium wall. This vascular network is composed of endothelial cells surrounded by smooth muscle cells, forming a network of branched tubular structures. The presence of vasculature significantly promotes the arrangement of the myocardium, enhances the organization of the cardiac cytoskeleton, and promotes the consistency and beating frequency of cardiac cells. The vasculature facilitates the growth and size increase of the cardiac organoids.

Abstract: Vascularized islets and their preparation method is presented. The vascularized islets are comprised of islet spheroids enclosed within a network of blood vessels. The vascular network is composed of endothelial cells surrounded by smooth muscle cells, forming a tubular vessel with branched structures. The presence of this vasculature significantly enhances the survival of the islets, improves the efficiency of pancreatic precursor cells, islet progenitor cells and R cells, and the increases the synthesis of insulin precursor C-peptide. The procedures for constructing vascularized islets are suitable for long-term in vitro culture of islets.

Abstract: Disclosed is a bending-resistant nerve catheter and a preparation method and application thereof, and relates to the technical field of tissue engineering materials. The catheter of the present application includes an inner layer, a middle layer and an outer layer, and each layer uses raw materials of biodegradable polymers; among them, the inner layer includes a smooth surface inner layer, an oriented microchannel inner layer or a fibrous inner layer, the middle layer is a fibrous middle layer with crossing angles, the middle layer is prepared by entangling micron fibers with a certain angular arrangement, and the outer layer is made of randomly entangled polymer fibers and is tightly bonded to the middle layer.

Abstract: A vascularized cardiac organoid with a chamber structure and its preparation method is presented. The cardiac organoids have a unique chamber structure, which includes a myocardium wall. Within the cardiac organoids, a vascular network is developed, enclosed within the myocardium wall. This vascular network is composed of endothelial cells surrounded by smooth muscle cells, forming a network of branched tubular structures. The presence of vasculature significantly promotes the arrangement of the myocardium, enhances the organization of the cardiac cytoskeleton, and promotes the consistency and beating frequency of cardiac cells. The vasculature facilitates the growth and size increase of the cardiac organoids.

Abstract: Vascularized islets and their preparation method is presented. The vascularized islets are comprised of islet spheroids enclosed within a network of blood vessels. The vascular network is composed of endothelial cells surrounded by smooth muscle cells, forming a tubular vessel with branched structures. The presence of this vasculature significantly enhances the survival of the islets, improves the efficiency of pancreatic precursor cells, islet progenitor cells and R cells, and the increases the synthesis of insulin precursor C-peptide. The procedures for constructing vascularized islets are suitable for long-term in vitro culture of islets.

Abstract: The present disclosure discloses a composite tubular material and a preparation method thereof. The tubular material is prepared from extracellular matrix and directionally arranged synthetic polymer fibers through compounding; the synthetic polymer fibers serve as an internal skeleton, a fiber diameter ranges from 1 ?m to 2000 ?m, a fiber angle ranges from 0° to 180°, and a wall thickness ranges from 1 ?m to 1000 ?m; and extracellular matrix components are obtained from human or animal tissue through decellularization. According to the present disclosure, the tubular material with bioactivity and excellent mechanical properties can be prepared from the synthetic polymer and the natural material extracellular matrix through compounding; the synthetic polymer fibers with controllable fiber angle and diameter serve as the internal skeleton of the tubular material, and accordingly the tubular material has the mechanical properties of resistance to bending and squeezing.

Abstract: The present disclosure discloses a preparation method and application of decellularized extracellular matrix tissue paper, and belongs to the technical field of regenerative medicine. The preparation method includes the following steps: (1) preparing decellularized extracellular matrix materials; (2) preparing tissue paper: homogenizing and stirring the prepared decellularized extracellular matrix materials, then, placing the obtained homogenates on a flat-bottom filter screen to filter out water, standing and air-drying the filtered homogenates, and freeze-drying the homogenates to obtain the tissue paper; (3) cross-linking the tissue paper: cross-linking the tissue paper in a cross-linking solution; and (4) freeze-drying or stacking and compacting the cross-linked tissue paper. According to the present disclosure, the decellularized extracellular matrix tissue paper with tissue specificity is prepared by a traditional paper-making technology combined with a freeze-drying technology.

Abstract: Disclosed is a bending-resistant nerve catheter and a preparation method and application thereof, and relates to the technical field of tissue engineering materials. The catheter of the present application includes an inner layer, a middle layer and an outer layer, and each layer uses raw materials of biodegradable polymers; among them, the inner layer includes a smooth surface inner layer, an oriented microchannel inner layer or a fibrous inner layer, the middle layer is a fibrous middle layer with crossing angles, the middle layer is prepared by entangling micron fibers with a certain angular arrangement, and the outer layer is made of randomly entangled polymer fibers and is tightly bonded to the middle layer.

Abstract: The invention relates to the complex of synthetic polymer and natural extracellular matrix for vascular grafts and their preparation methods. The components of biodegradable synthetic polymer in the preparation process can be chosen with different material proportions. The scaffolds with different fiber diameters, different fiber arrangements, different pore sizes and different pore structures can be prepared by electro-spinning, wet-spinning, melt-spinning, 3D printing, pouring, phase separation, particle leaching and other technologies. Among them, the natural extracellular matrix components come from a wide range of sources such as vascular tissues from different kinds of animals including arteries and veins of pigs and cattle or vascular tissues from human donors including umbilical cord vessels, etc. And its composition and content can be flexibly adjusted according to the demand.

Abstract: A method for preparing a carbonized resin DNA immunoadsorbent, which uses styrene, acrylonitrile, divinylbenzene, toluene, liquid paraffin, benzoyl peroxide and water solution of polyvinyl alcohol as raw materials, produces a DNA immunoadsorbent with 0.3-0.5 mg DNA per milliliter of resin using two-staged procedures for preparation of first carbonized resin, and secondly carbonized resin DNA immunoadsorbent. The resultant immunoadsorbent is high in adsorbent capacity, low in production cost, and can be synthesized without pyrogens. It therefore satisfies the demands of medical application for the treatment of systemic lupus erythematosus by immunoadsorption.