Abstract: The invention relates to a method for producing an unshrunken tissue equivalent. Said method is comprising in that: a mixture is produced that contains at least one element of an extracellular matrix; at least one pluridimensional medium is soaked with said mixture; from the components of the mixture, a lattice comprising at least one element of an extracellular matrix is produced, at least at the medium; at least part of the components of said mixture is attached to the structure of the medium; the shrinking of at least the lattice is prevented and at least said lattice is tensioned on said medium; fibroblasts are integrated into the lattice and at least one cell culture of said fibroblasts is carried out, at least in the lattice. The invention also relates to a method for producing a skin equivalent that comprises at least one dermal equivalent formed by an unshrunken tissue equivalent and at least one epidermal equivalent.

Abstract: A diffusion insert (1) for a membrane (5), includes a cup (2) defining a donor compartment (20) receiving a donor solution, the base thereof defining an axial port (22) sealed by the membrane (5), a clamping element (3) to pin down the membrane (5), an element for axially locking (4) the clamping element (3), a deformation element (6) capable of deforming the membrane (5) and of protecting the lateral movement directly tangential to the deformation element (6), characterized in that the insert includes a return element (7) for deforming and radially stretching the membrane (5) in a controlled manner. A kit, a cell, a diffusion unit, and a diffusion method for implementing the diffusion insert are also disclosed.

Abstract: The invention relates to a method for producing an unshrunken tissue equivalent. Said method is comprising in that: a mixture is produced that contains at least one element of an extracellular matrix; at least one pluridimensional medium is soaked with said mixture; from the components of the mixture, a lattice comprising at least one element of an extracellular matrix is produced, at least at the medium; at least part of the components of said mixture is attached to the structure of the medium; the shrinking of at least the lattice is prevented and at least said lattice is tensioned on said medium; fibroblasts are integrated into the lattice and at least one cell culture of said fibroblasts is carried out, at least in the lattice. The invention also relates to a method for producing a skin equivalent that comprises at least one dermal equivalent formed by an unshrunken tissue equivalent and at least one epidermal equivalent.