Abstract: Vertically cut a “quasi-upper buffer chamber” (such as the part 11 of US 8808520) into 2 pieces of U-shaped doorframes, and then fix them onto the 2 opposite side walls of a transparent ark, so as to divide the ark into 3 compartments. Position a tubular telescopic urging mechanism into the middle compartment, and then use it to push 2 vertical slice gel cassettes to resting against the 2 door-frames respectively, so as to convert the 3 compartments into 3 watertight compartments. Wherein, the middle one is the lower buffer chamber, while the other 2 are the 2 upper buffer chambers of the present anti-traditional vertical slice gel electrophoresis cell.

Abstract: Use a pair of U-shaped doorframe (with U-shaped rubber sealing gaskets affixed along them) to divide the inside of a transparent ark into 3 compartments. Develop out a telescopic pushing tool. Place it into the middle compartment, and then use it to force 2 pieces of vertical slice gel cassette to rest against the 2 door frames respectively, so as to transform the 3 compartments into 3 water-tight compartments. Wherein, the 2 side 2 are the 2 upper buffer chambers of the present electrophoresis cell. This kind of electrophoresis cell has only 2 necessary components, and it can be made to be compatible with all of those so called mini gel cassettes with different thicknesses and/or heights.

Abstract: In the present innovated vertical slice gel electrophoresis cell, at least one piece of large diameter screw urging ring is held to face to one U-shaped side opening of an upper buffer chamber, but an interval is left therebetween. After inserting a gel cassette into the interval, turning tight the screw urging ring can force at least one piece of gel cassette to join the upper buffer chamber tightly, no matter how thick the cassette is. In addition, some other materials are found also suitable for absorbing the Joule-heat after sealing them into dielectric shells respectively.

Abstract: In the present innovated vertical slice gel electrophoresis cell, at least one piece of large diameter screw urging ring is held to face to one U-shaped side opening of an upper buffer chamber, but an interval is left therebetween. After inserting a gel cassette into the interval, turning tight the screw urging ring can force at least one piece of gel cassette to join the upper buffer chamber tightly, no matter how thick the cassette is. In addition, some other materials are found also suitable for absorbing the Joule-heat after sealing them into dielectric shells respectively.

Abstract: In the present vertical slab-gel electrophoresis instrument, all the vertical slab gel cassettes comprise un-notched rectangular sidewalls and flanged spacer strips, but still can form a U-notched upper opening with an even rim for each cassette; one type of cassette can undergo gel casting with an electrophoresis cell, while another two types of cassettes can be used to form a multiple-cassette electrophoresis cell. The present invention can also incorporate a swing-frame chosen for urging a cassette to join an upper buffer chamber, provides an improved cooling device such that there is no need for the use of exogenous coolant, and also provides simple methods to enable cassettes to be tightly enclosed within membrane pouches for gel casting.

Abstract: In the present innovations on vertical slab gel electrophoresis cell and method, 6 embodiments embodied 3 different mechanisms for urging the cassettes to abut the upper buffer chamber, every of them has its merit; a dual-gel cell allows not to block up the other side opening when one gel runs in it; all cassettes are formed by rectangular sidewalls and fringed spacer strips, but still can form a U-notched upper opening with even rim for every cassette, 2 kind cassettes can undergo the gel casting within the electrophoresis cell, other 2 kind cassettes can form an unlimited cassette/UBC complex; a cooling device needs not exogenous coolant; disclosed 4 convenient methods can snugly encase almost all kind cassettes into membrane pouches for perform homogeneous or gradient gel casting.

Abstract: A gradient generator comprising plural vessels of identical height but varied in cross section filled with compositional different liquids, having inlets for filling and outlets connected to a common discharge tube for discharge and delivering; but said plural vessels are formed by plural pieces of non-rigid bags with different profiles arranged side by side and sandwiched within a clearance of an erect parallel plate pair, and has an extrusion means at bottom to extrude and press flat of said filled plural non-rigid bag vessels parallel from their bottom toward the top when said outlets connected to said common discharge tube is at top.