Abstract: The present application discloses an intestine-targeted drug delivery composition, comprising in sequence, from inside to outside, (1) a microsphere core and a pH-independent sustained-release layer containing an active ingredient, or a microsphere containing an active ingredient and a pH-independent sustained-release layer, or a microsphere containing an active ingredient and a pH-independent sustained-release material, (2) a pH-dependent coating layer, and (3) a time-dependent pulsatile controlled-release layer. The intestine-targeted drug delivery composition of the present application delivers the active ingredient to intestinal tissues (especially colon tissue) for release, reduces the systemic blood concentration of the active ingredient, and improves the pharmacokinetic characteristics and concentration of the active ingredient in intestinal tissues. The composition is suitable for treating intestinal diseases, such as inflammatory bowel diseases including Crohn's disease and ulcerative colitis.