Abstract: Fluorescent nanosensors for extracellular ion concentration measurements are disclosed herein. More specifically, a fluorescent nanosensor for extracellular ion measurements comprising a photoluminescent nanostructure disposed on a substrate surface is disclosed. The photoluminescent nanostructure comprises a fluorescent metallic core-silica shell containing nanoparticle, wherein the fluorescent silica shell comprises a fluorophore dispersed therein. The nanosensor emits a fluorescence emission in function of the extracellular ion concentration.

Abstract: A patterned capillary device comprising a pattern on an inner surface is described herein. More specifically, the patterned capillary device comprises a wall having an outer surface and an inner surface, the wall defining a fluidic channel. A substantially opaque film covers the inner surface of the wall, the opaque film comprising a plurality of substantially transparent segments defining a pattern. A process for fabricating a pattern formed of opaque and transparent portions on an inner surface of a capillary device is also described herein.

Abstract: Fluorescent nanosensors for extracellular ion concentration measurements are disclosed herein. More specifically, a fluorescent nanosensor for extracellular ion measurements comprising a photoluminescent nanostructure disposed on a substrate surface is disclosed. The photoluminescent nanostructure comprises a fluorescent metallic core-silica shell containing nanoparticle, wherein the fluorescent silica shell comprises a fluorophore dispersed therein. The nanosensor emits a fluorescence emission in function of the extracellular ion concentration.

Abstract: A patterned capillary device comprising a pattern on an inner surface is described herein. More specifically, the patterned capillary device comprises a wall having an outer surface and an inner surface, the wall defining a fluidic channel. A substantially opaque film covers the inner surface of the wall, the opaque film comprising a plurality of substantially transparent segments defining a pattern. A process for fabricating a pattern formed of opaque and transparent portions on an inner surface of a capillary device is also described herein.

Abstract: The invention relates to methods and reagents for detecting minute amounts of targets having affinity for nucleic acid. The present invention more particularly relates to target detection using aggregates of cationic polymer chains and nucleic acid capture probes linked to particles, such as controllable mobility particles.

Abstract: A method of detecting a fluorescence signal emitted by fluorophores bound to particles confined in a particle trap, includes an objective lens having a focal plane, which is normally the focal plane for incident collimated light. The particle trap is typically located in the focal plane, and a beam of excitation light is directed via the objective lens onto the confined particles in the trap. The excitation light is in the form of a divergent beam coming to focus at a plane displaced from the focal plane. The divergent beam has a spot diameter at the focal plane determined by the divergence of the beam. The fluorescent light emitted by the fluorophores is detected with a confocal detector.

Abstract: The invention relates to methods and reagents for detecting minute amounts of targets having affinity for nucleic acid. The present invention more particularly relates to target detection using aggregates of cationic polymer chains and nucleic acid capture probes linked to particles, such as controllable mobility particles.