Abstract: The present invention provides a device and method for the purification and separation of substances. The purification device comprises a sample holder comprising a sample chamber and a column module. The column module is securable to the sample holder and is packed with chromatography medium having a special affinity for attracting a given substance. A variety of mediums can be used in the capsule of the purification device, each containing different medium types. The column modules may be prepacked by the manufacturer or may be provided empty so that a user defined medium may be inserted into the capsule by the user. With the column module and sample loaded, pressure may be applied to move the sample through the module to by means such as centrifuging in order to separate the selected substance from the sample.