Patents by Inventor Denis Pristinski
Denis Pristinski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200371329Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.Type: ApplicationFiled: November 7, 2019Publication date: November 26, 2020Inventors: Gilad ALMOGY, Nathan BECKETT, Nathan CASWELL, Jacob A. WOLF, Gene POLOVY, Denis PRISTINSKI
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Publication number: 20200326327Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: ApplicationFiled: April 29, 2020Publication date: October 15, 2020Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
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Publication number: 20200150410Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.Type: ApplicationFiled: January 16, 2020Publication date: May 14, 2020Applicant: Bio-Rad Laboratories, Inc.Inventors: Denis PRISTINSKI, David P. STUMBO
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Patent number: 10551603Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.Type: GrantFiled: July 21, 2017Date of Patent: February 4, 2020Assignee: Bio-Rad Laboratories, Inc.Inventors: Denis Pristinski, David P. Stumbo
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Publication number: 20190257687Abstract: System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element including a light guide and an exit window, and at least one detector.Type: ApplicationFiled: May 6, 2019Publication date: August 22, 2019Applicant: Bio-Rad Laboratories, Inc.Inventor: Denis PRISTINSKI
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Patent number: 10344328Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.Type: GrantFiled: May 8, 2018Date of Patent: July 9, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
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Publication number: 20190153520Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: ApplicationFiled: May 8, 2018Publication date: May 23, 2019Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
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Publication number: 20190153531Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.Type: ApplicationFiled: May 8, 2018Publication date: May 23, 2019Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
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Patent number: 10281320Abstract: System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element, and a detector. The optical element may include a light guide having a shaft and a tip, with the tip forming a beveled end of the light guide. The optical element may extend into the beam, such that the tip and at least a portion of the shaft are located inside the beam, and a window of the optical element is located outside the beam. Light of the beam incident on the tip may be transmitted longitudinally through the light guide and out the window, while light of the beam incident on the shaft may be transmitted transversely through the shaft and remains in the beam downstream. The detector may be configured to detect light received from the window.Type: GrantFiled: December 29, 2016Date of Patent: May 7, 2019Assignee: Bio-Rad Laboratories, Inc.Inventor: Denis Pristinski
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Patent number: 10273528Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: GrantFiled: May 8, 2018Date of Patent: April 30, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
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Patent number: 10267790Abstract: Provided are systems for biological sample processing and analysis. A system can comprise a substrate configured to rotate. The substrate can comprise an array configured to immobilize a biological analyte. A fluid flow unit comprising a fluid channel can be configured to dispense a solution comprising a plurality of probes. The solution may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector in optical communication with the array can be configured to detect one or more signals from the at least one probe coupled to the biological analyte.Type: GrantFiled: May 8, 2018Date of Patent: April 23, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
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Publication number: 20190002956Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: ApplicationFiled: June 28, 2018Publication date: January 3, 2019Inventors: David P. STUMBO, George CARMAN, Steve HOBBS, Anthony J. MAKAREWICZ, JR., Dmitri SIMONIAN, David GLADE, Joshua OEN, Denis PRISTINSKI, John DZENITIS
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Patent number: 10060847Abstract: Systems and methods for detecting and processing signals from particles. In an exemplary method, particles may be passed through a zone of a channel, while the zone is irradiated with light. Interaction of the light with the particles may deflect light and induce photoluminescence. A deflection signal and a photoluminescence signal may be detected from the zone. Particle waveforms may be identified in the deflection signal. At least a subset of the particle waveforms may be double-peak waveforms including a pair of peaks corresponding to a particle entering and exiting the zone. Amplitudes may be obtained from the photoluminescence signal. The amplitudes may correspond to respective particles and their particle waveforms, and at least a subset of the amplitudes may correspond to the double-peak waveforms. Individual particles may be assigned as positive or as negative for an analyte based on the corresponding amplitudes.Type: GrantFiled: December 29, 2016Date of Patent: August 28, 2018Assignee: Bio-Rad Laboratories, Inc.Inventors: Yunfeng Ling, David P. Stumbo, George Carman, Denis Pristinski
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Patent number: 9846111Abstract: Optical detection system and method for particles. In exemplary embodiments, the system may comprise a channel, a light source configured to generate light, and one or more optical elements configured to focus a beam of the light on an irradiation zone within the channel. The system also may comprise a mask operatively disposed in an optical path between the light source and the channel. The mask may be configured to block a portion of the beam, thereby producing a shadow region. The system further may comprise a detector configured to detect light deflected from the beam into the shadow region by interaction with a particle passing through the irradiation zone.Type: GrantFiled: December 29, 2016Date of Patent: December 19, 2017Assignee: Bio-Rad Laboratories, Inc.Inventor: Denis Pristinski
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Publication number: 20170322401Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.Type: ApplicationFiled: July 21, 2017Publication date: November 9, 2017Inventors: Denis Pristinski, David P. Stumbo
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Patent number: 9726871Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.Type: GrantFiled: January 31, 2014Date of Patent: August 8, 2017Assignee: Bio-Rad Laboratories, Inc.Inventors: Denis Pristinski, David P. Stumbo
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Publication number: 20170191924Abstract: Optical detection system and method for particles. In exemplary embodiments, the system may comprise a channel, a light source configured to generate light, and one or more optical elements configured to focus a beam of the light on an irradiation zone within the channel. The system also may comprise a mask operatively disposed in an optical path between the light source and the channel. The mask may be configured to block a portion of the beam, thereby producing a shadow region. The system further may comprise a detector configured to detect light deflected from the beam into the shadow region by interaction with a particle passing through the irradiation zone.Type: ApplicationFiled: December 29, 2016Publication date: July 6, 2017Inventor: Denis Pristinski
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Publication number: 20170191923Abstract: Systems and methods for detecting and processing signals from particles. In an exemplary method, particles may be passed through a zone of a channel, while the zone is irradiated with light. Interaction of the light with the particles may deflect light and induce photoluminescence. A deflection signal and a photoluminescence signal may be detected from the zone. Particle waveforms may be identified in the deflection signal. At least a subset of the particle waveforms may be double-peak waveforms including a pair of peaks corresponding to a particle entering and exiting the zone. Amplitudes may be obtained from the photoluminescence signal. The amplitudes may correspond to respective particles and their particle waveforms, and at least a subset of the amplitudes may correspond to the double-peak waveforms. Individual particles may be assigned as positive or as negative for an analyte based on the corresponding amplitudes.Type: ApplicationFiled: December 29, 2016Publication date: July 6, 2017Inventors: Yunfeng Ling, David P. Stumbo, George Carman, Denis Pristinski
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Publication number: 20170184448Abstract: System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element, and a detector. The optical element may include a light guide having a shaft and a tip, with the tip forming a beveled end of the light guide. The optical element may extend into the beam, such that the tip and at least a portion of the shaft are located inside the beam, and a window of the optical element is located outside the beam. Light of the beam incident on the tip may be transmitted longitudinally through the light guide and out the window, while light of the beam incident on the shaft may be transmitted transversely through the shaft and remains in the beam downstream. The detector may be configured to detect light received from the window.Type: ApplicationFiled: December 29, 2016Publication date: June 29, 2017Inventor: Denis Pristinski
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Publication number: 20140218719Abstract: Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.Type: ApplicationFiled: January 31, 2014Publication date: August 7, 2014Applicant: Bio-Rad Laboratories, Inc.Inventors: Denis Pristinski, David P. Stumbo