Patents by Inventor Dennis J. Sawchuk

Dennis J. Sawchuk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9657357
    Abstract: The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: May 23, 2017
    Assignee: CHROMOCELL CORPORATION
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jessica C. Langer
  • Publication number: 20170052170
    Abstract: The invention relates to novel cells and cell lines, and methods for making and using them.
    Type: Application
    Filed: August 8, 2016
    Publication date: February 23, 2017
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Purvi Manoj Shah
  • Publication number: 20150315554
    Abstract: Disclosed herein are cells and cell lines that stably express CFTR and methods for using those cells and cell lines. The invention also includes techniques for creating these cells and cell lines. The cells and cell lines of this invention are physiologically relevant. They are highly sensitive and provide consistent and reliable results in cell-based assays.
    Type: Application
    Filed: November 24, 2014
    Publication date: November 5, 2015
    Inventors: Kambiz Shekdar, Jessica Langer, Srinivasan P. Venkatachalan, Dennis J. Sawchuk
  • Publication number: 20150299782
    Abstract: Methods of isolating cells or generating cell lines comprising the step of exposing the cells to signaling probes that produce a signal upon hybridization to a target sequence, as well as methods of quantifying the level of expression of an RNA of interest, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. Methods for isolating a plurality of cells encoding a plurality of different RNAs associated with a same nucleic acid tag sequence, comprising the step of exposing the cells to a same signaling probe that produces a detectable signal upon hybridization to the same nucleic acid tag sequence, are also provided. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures may be used in the above methods.
    Type: Application
    Filed: November 6, 2014
    Publication date: October 22, 2015
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jason M. Montez
  • Publication number: 20150141289
    Abstract: The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 21, 2015
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jessica C. Langer
  • Patent number: 8945848
    Abstract: The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: February 3, 2015
    Assignee: Chromocell Corporation
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jessica C. Langer
  • Patent number: 8916503
    Abstract: Methods of isolating cells or generating cell lines comprising the step of exposing the cells to signaling probes that produce a signal upon hybridization to a target sequence, as well as methods of quantifying the level of expression of an RNA of interest, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. Methods for isolating a plurality of cells encoding a plurality of different RNAs associated with a same nucleic acid tag sequence, comprising the step of exposing the cells to a same signaling probe that produces a detectable signal upon hybridization to the same nucleic acid tag sequence, are also provided. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures may be used in the above methods.
    Type: Grant
    Filed: February 17, 2005
    Date of Patent: December 23, 2014
    Assignee: Chromocell Corporation
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jason M. Montez
  • Publication number: 20120276572
    Abstract: The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
    Type: Application
    Filed: July 30, 2010
    Publication date: November 1, 2012
    Applicant: CHROMOCELL CORPORATION
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jessica C. Langer
  • Publication number: 20120058918
    Abstract: Disclosed herein are cells and cell lines that stably express CFTR and methods for using those cells and cell lines. The invention also includes techniques for creating these cells and cell lines. The cells and cell lines of this invention are physiologically relevant. They are highly sensitive and provide consistent and reliable results in cell-based assays.
    Type: Application
    Filed: February 1, 2010
    Publication date: March 8, 2012
    Applicant: CHROMOCELL CORPORATION
    Inventors: Kambiz Shekdar, Jessica Langer, Srinivasan P. Venkatachalan, Dennis J. Sawchuk
  • Publication number: 20090106853
    Abstract: The present invention relates to methods of isolating cells or generating cell lines using signaling probes that produce a signal upon hybridization to a target sequence. Other methods that utilize the signaling probe include methods of quantifying the level of RNA expression, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. The invention also provides protease probes. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures are provided.
    Type: Application
    Filed: February 17, 2005
    Publication date: April 23, 2009
    Inventors: Kambiz Shekdar, Dennis J. Sawchuk, Jason M. Montez