Patents by Inventor Dennis Jay Eastburn
Dennis Jay Eastburn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11891666Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: GrantFiled: April 6, 2021Date of Patent: February 6, 2024Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Patent number: 11781129Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: GrantFiled: October 21, 2019Date of Patent: October 10, 2023Assignee: MISSION BIO, INC.Inventor: Dennis Jay Eastburn
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Publication number: 20230109667Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.Type: ApplicationFiled: March 17, 2022Publication date: April 6, 2023Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
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Publication number: 20220275427Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.Type: ApplicationFiled: March 17, 2022Publication date: September 1, 2022Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
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Patent number: 11312990Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.Type: GrantFiled: April 23, 2020Date of Patent: April 26, 2022Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
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Patent number: 11203787Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: GrantFiled: October 18, 2018Date of Patent: December 21, 2021Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20210388446Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: ApplicationFiled: April 6, 2021Publication date: December 16, 2021Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20210246488Abstract: The disclosure relates to methods and compositions for evaluating emulsion uniformity. In an exemplary embodiment, the disclosure provides a method for evaluating a quality characteristic of a droplet. The method includes the steps of: encapsulating a plurality of polynucleotides in a droplet, wherein the plurality of polynucleotides comprises at least one species of oligonucleotide; tagging the polynucleotides with a label that identifies the polynucleotides as arising from the droplet; counting a number of species of oligonucleotide tagged with the label; and determining a quality characteristic of the droplet based on the number of species of oligonucleotide tagged with the label. The oligonucleotide may include a first nucleic acid segment and a second nucleic acid segment, wherein the first nucleic acid segment comprises a plurality of random nucleotides; and the second nucleic acid segment comprises a conserved region common to the plurality of oligonucleotides.Type: ApplicationFiled: August 14, 2018Publication date: August 12, 2021Applicant: Mission Bio, Inc.Inventors: Sebastian Treusch, Adam R. Sciambi, Maurizio Pellegrino, Dennis Jay Eastburn
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Patent number: 11001896Abstract: The disclosed embodiments generally relate to a method and system to synthesize a target molecule within a droplet. In an exemplary embodiment, a first microfluidic device configured to contact a polynucleotide-containing component from a sample with lysis reagents to form a first droplet. The lysis reagents include an enzyme having protease activity. The first droplet is encapsulated with an immiscible carrier fluid. A collection reservoir is provided to receive and incubate the first droplet for a first duration of time. The first duration of time is sufficient to inactivate the enzyme of the lysis reagent. A second microfluidic device is provided to receive the first droplet and add nucleic acid synthesis reagent to thereby form a second nucleic acid synthesis droplet in the immiscible carrier fluid. Finally, a reaction chamber is provided to synthesize the target polynucleotide within the second nucleic acid synthesis droplet.Type: GrantFiled: April 11, 2019Date of Patent: May 11, 2021Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20210002697Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.Type: ApplicationFiled: April 23, 2020Publication date: January 7, 2021Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
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Patent number: 10745762Abstract: The disclosed embodiments relate to method, system and apparatus for synthesizing a target polynucleotide within a droplet. In an exemplary embodiment, the disclosure provides a method of synthesizing a target polynucleotide. The method includes the steps of: contacting a polynucleotide-containing component from a sample with lysis reagents in a droplet, the lysis reagents comprising an enzyme having protease activity, wherein the droplet is encapsulated with an immiscible carrier fluid; moving the droplet into a collection reservoir; incubating the droplet in the collection reservoir for a first duration and then inactivating the enzyme having protease activity; adding to the droplet a nucleic acid synthesis reagent to form a nucleic acid synthesis droplet in the immiscible carrier fluid to form a nucleic acid droplet; and synthesizing the target polynucleotide within the nucleic acid synthesis droplet.Type: GrantFiled: February 19, 2019Date of Patent: August 18, 2020Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Patent number: 10697007Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.Type: GrantFiled: June 25, 2015Date of Patent: June 30, 2020Assignee: The Regents of the University of CaliforniaInventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
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Publication number: 20200071692Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: ApplicationFiled: October 21, 2019Publication date: March 5, 2020Applicant: Mission Bio, Inc.Inventor: Dennis Jay Eastburn
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Patent number: 10501739Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: GrantFiled: October 24, 2018Date of Patent: December 10, 2019Assignee: Mission Bio, Inc.Inventor: Dennis Jay Eastburn
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Publication number: 20190329209Abstract: Disclosed herein are methods and compositions for the emulsification of solid supports in deformable gel beads. The methods and compositions provided herein may be used in microfluidic systems and devices. In some aspects of the disclosure, deformable gel beads containing solid supports may be paired with single cell entities. The methods and compositions provided herein may be suitable for single cell analysis, including, but not limited to, labeling single cells or components thereof for downstream analysis.Type: ApplicationFiled: June 13, 2017Publication date: October 31, 2019Applicant: Mission Bio, Inc.Inventors: Adam R. Sciambi, Dennis Jay Eastburn, Sebastian Treusch
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Publication number: 20190330701Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: ApplicationFiled: April 11, 2019Publication date: October 31, 2019Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20190241965Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: ApplicationFiled: October 18, 2018Publication date: August 8, 2019Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20190169700Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.Type: ApplicationFiled: February 19, 2019Publication date: June 6, 2019Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
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Publication number: 20190112655Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a microfluidic apparatus is used to provide a rapid and cost-effective targeted genomic sequencing of thousands of cells in parallel. The targeted sequencing can be directed for residual disease detection.Type: ApplicationFiled: October 18, 2018Publication date: April 18, 2019Applicant: Mission Bio, Inc.Inventors: Dennis Jay Eastburn, Adam R. Sciambi, Maurizio Pellegrino
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Publication number: 20190112595Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: ApplicationFiled: October 24, 2018Publication date: April 18, 2019Applicant: Mission Bio, Inc.Inventor: Dennis Jay Eastburn