Abstract: Sanitising compositions are provided for use in combination with cleaning compositions, cleaning and sanitization protocols with such compositions and applications of such sanitising compositions. The compositions comprise (a) biocidal component comprising one or more non-polymeric biguanides and analogs thereof, and one or more quaternary ammonium compounds and a formulation comprising (b) one or more cationic detergents having at least one unsubstituted alkyl group of 8 or more carbon atoms, (c) one or more chelating agents, and (d) an amine component comprising a mixture of at least one alkanolamine and at least one bis(aminoalkyl)alkylamine. The composition may be further enhanced by the incorporation of a mixture of surfactants comprises as a first component one or more non-ionic surfactants and as a second component one or more amphoteric surfactants. The composition is particularly effective when used in combination with a cleaning composition comprising the same mixture of surfactants.