Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: This invention relates generally to systems for handling biological fluids such as blood and more particularly relates to apparatus for maintaining a constant flow rate of fluid by a peristaltic pump when the pressure at the input of such a pump goes negative or positive. More specifically, the invention uses a microprocessor which provides an output signal representative of the incremental change in pump speed required when the pump input experiences a negative or positive pressure regime.The microprocessor output is based on a sensed input pump pressure, P.sub.o, a pressure, P.sub.i, which is the pressure at which extensions of the linearly decreasing portions of the pump characteristic curves intersect the pressure axis on a plot of Pressure vs Flow Rate for a given pump and a desired flow rate, Q.sub.o. The resulting output is applied to a stepper motor via a programmable timer and driver amplifier.