Patents by Inventor Deping Xu
Deping Xu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11655208Abstract: The present disclosure discloses a 1-carboxy-2-hydroxy-3-iminopropane and an extraction method thereof, and belongs to the technical fields of food, health food and medicine.Type: GrantFiled: December 13, 2021Date of Patent: May 23, 2023Assignee: JIANGNAN UNIVERSITYInventors: Xiulan Sun, Yongli Ye, Qianqian Hong, Deping Xu, Yinzhi Zhang, Shuxiang Geng
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Patent number: 11384109Abstract: The disclosure discloses a method for separating phenolic acid glucoside compounds from Diaphragma juglandis Fructus, belonging to the technical field of food. The method includes the following steps: (1) extraction: crushing Diaphragma juglandis Fructus, and then extracting by using ethanol to obtain walnut ethanol extract; and (2) separation: performing column chromatography on the walnut ethanol extract obtained in step (1), sequentially eluting by using ethanol of different concentrations, purifying and eluting repeatedly, and performing separation to obtain phenolic acid glucoside compounds. The phenolic acid glucoside compounds are 2-hydroxyl-5-methoxylphenyl-O-?-D-glucopyranoside, 6-hydroxylnaphthyl-1,2-di-O-?-D-glucopyranoside and 2,6-dihydroxynaphthyl-O-?-D-glucopyranoside. Pharmacological experiments confirm that the three compounds have good antioxidant activity.Type: GrantFiled: October 21, 2021Date of Patent: July 12, 2022Assignee: Jiangnan UniversityInventors: Xiulan Sun, Yida Xu, Deping Xu, Yinzhi Zhang
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Publication number: 20220098144Abstract: The present disclosure discloses a 1-carboxy-2-hydroxy-3-iminopropane and an extraction method thereof, and belongs to the technical fields of food, health food and medicine.Type: ApplicationFiled: December 13, 2021Publication date: March 31, 2022Inventors: Xiulan SUN, Qianqian HONG, Deping XU, Yinzhi ZHANG, Shuxiang GENG
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Publication number: 20220041639Abstract: The disclosure discloses a method for separating phenolic acid glucoside compounds from Diaphragma juglandis Fructus, belonging to the technical field of food. The method includes the following steps: (1) extraction: crushing Diaphragma juglandis Fructus, and then extracting by using ethanol to obtain walnut ethanol extract; and (2) separation: performing column chromatography on the walnut ethanol extract obtained in step (1), sequentially eluting by using ethanol of different concentrations, purifying and eluting repeatedly, and performing separation to obtain phenolic acid glucoside compounds. The phenolic acid glucoside compounds are 2-hydroxyl-5-methoxylphenyl-O-?-D-glucopyranoside, 6-hydroxylnaphthyl-1,2-di-O-?-D-glucopyranoside and 2,6-dihydroxynaphthyl-O-?-D-glucopyranoside. Pharmacological experiments confirm that the three compounds have good antioxidant activity.Type: ApplicationFiled: October 21, 2021Publication date: February 10, 2022Inventors: Xiulan Sun, Yida Xu, Deping Xu, Yinzhi Zhang
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Patent number: 9273321Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: GrantFiled: October 17, 2014Date of Patent: March 1, 2016Assignee: PIONEER HI BRED INTERNATIONAL INC.Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20150037807Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: ApplicationFiled: October 17, 2014Publication date: February 5, 2015Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20140007300Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.Type: ApplicationFiled: February 1, 2013Publication date: January 2, 2014Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MYOENG-JE CHO, XINLI E. WU, DEPING XU, ZUO-YU ZHAO
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Patent number: 8389807Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.Type: GrantFiled: December 30, 2009Date of Patent: March 5, 2013Assignee: Pioneer Hi-Bred International, Inc.Inventors: Zuo-Yu Zhao, Xinli E. Wu, Myoeng-Je Cho, Deping Xu
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Publication number: 20120329674Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.Type: ApplicationFiled: December 6, 2011Publication date: December 27, 2012Applicant: PIONEER HI BRED INTERNATIONAL INCInventors: Marc C. Albertsen, Susan J. Martino-Catt, Xiaomu Niu, Igor Cunha Oliveira, Dwight Tomes, Haiyin Wang, Deping Xu
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Publication number: 20120071348Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.Type: ApplicationFiled: March 28, 2011Publication date: March 22, 2012Applicant: PIONEER HI BRED INTERNATIONAL INCInventors: MARC C. ALBERTSEN, SUSAN J. MARTINO-CATT, XIAOMU NIU, IGOR CUNHA OLIVEIRA, DWIGHT TOMES, HAIYIN WANG, DEPING XU
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Publication number: 20110165561Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: ApplicationFiled: December 23, 2010Publication date: July 7, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20110160087Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.Type: ApplicationFiled: December 30, 2009Publication date: June 30, 2011Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZUO-YU ZHAO, XINLI E. WU, MYOENG-JE CHO, DEPING XU
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Patent number: 7928287Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.Type: GrantFiled: February 13, 2003Date of Patent: April 19, 2011Assignee: Pioneer Hi-Bred International, Inc.Inventors: Dwight T. Tomes, Deping Xu, Haiyin Wang, Xiaomu Niu, Igor C. Oliveira
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Patent number: 7544857Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.Type: GrantFiled: October 30, 2006Date of Patent: June 9, 2009Assignees: Pioneer Hi-Bred International, Inc., The Curators of the University of MissouriInventors: Dilbag Multani, Xiaomu Niu, Dwight T. Tomes, Haiyin Wang, Deping Xu
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Patent number: 7317141Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.Type: GrantFiled: March 22, 2006Date of Patent: January 8, 2008Assignee: Pioneer Hi-Bred International, Inc.Inventors: Sobhana Sivasankar, Timothy G. Helentjaris, Deping Xu, Anna Lyznik
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Patent number: 7253000Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.Type: GrantFiled: April 26, 2005Date of Patent: August 7, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Sobhana Sivasankar, Timothy J. Helentjaris, Deping Xu
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Publication number: 20070044180Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.Type: ApplicationFiled: October 30, 2006Publication date: February 22, 2007Applicants: Pioneer Hi-Bred International, Inc., The Curators of the University of MissouriInventors: Dilbag Multani, Xiaomu Niu, Dwight Tomes, Haiyin Wang, Deping Xu
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Patent number: 7148401Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.Type: GrantFiled: August 31, 2004Date of Patent: December 12, 2006Assignees: Pioneer Hi-Bred International, Inc., The Curators of the University of MissouriInventors: Dilbag Multani, Xiaomu Niu, Dwight T. Tomes, Haiyin Wang, Deping Xu
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Publication number: 20060162027Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.Type: ApplicationFiled: March 22, 2006Publication date: July 20, 2006Inventors: Sobhana Sivasankar, Timothy Helentjaris, Deping Xu
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Publication number: 20060026716Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.Type: ApplicationFiled: April 26, 2005Publication date: February 2, 2006Applicant: Pioneer Hi-Bred International, Inc.Inventors: Sobhana Sivasankar, Timothy Helentjaris, Deping Xu