Abstract: The present disclosure provides a method for fragmenting cDNA on a support, where such fragmenting allows for application of short-read sequencing to longer cDNAs. In this method, the fragments of a cDNA molecule each receive the same unique molecular identifier (UMI), thereby allowing assembly of cDNA sequences from multiple short read sequences. Thus, the sequencing, transcript reconstruction and transcript analysis may be done at single molecule resolution.

Abstract: Provided herein is a method for producing a population of symmetrically barcoded transposomes, i.e., transposomes that are loaded with a pair of identical double-stranded adapters. In some embodiments, this method may comprise amplifying a template that contains a randomized sequence and a transposon end sequence on a solid support by bridge polymerase chain reaction (PCR) to produce uniquely barcoded clusters of single-stranded amplification products that are tethered to the support, processing the single-stranded amplification products so that the transposon end sequence is double-stranded and at the end of the products, and adding transposase to the support under conditions by which the transposase binds to the double-stranded transposon end sequences, to produce population of symmetrically barcoded transposomes.

Abstract: A particle isolation apparatus 100 for isolating particles from a suspension sample, includes a droplet dispenser device 10 for collecting the suspension sample from a carrier substrate 20 and for dispensing droplets onto a target substrate 30, a mechanical pump device 40 being coupled with the droplet dispenser device 10 for loading a dilution liquid into the droplet dispenser device 10 and for aspirating a first portion of the suspension sample into the droplet dispenser device 10, and a syphon pump device 50 being coupled with the droplet dispenser device 10 and being arranged for aspirating a second portion of the suspension sample into the droplet dispenser device 10. Preferably, the droplet dispenser device 10 is configured for dispensing single particle droplets on the target substrate 30. Furthermore, a method of isolating particles from a suspension sample is described.

Abstract: The present disclosure generally relates to spatial detection of a nucleic acid, such as a genomic DNA or a RNA transcript, in a cell comprised in a tissue sample. The present disclosure provides methods for detecting and/or analyzing nucleic acids, such as chromatin or RNA transcripts, so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. The present disclosure thus provides a process for performing “spatial transcriptomics” or “spatial genomics,” which enables the user to determine simultaneously the expression pattern, or the location/distribution pattern of the genes expressed or genes or genomic loci present in a single cell while retaining information related to the spatial location of the cell within the tissue architecture.

Abstract: A particle isolation apparatus 100 for isolating particles from a suspension sample, includes a droplet dispenser device 10 for collecting the suspension sample from a carrier substrate 20 and for dispensing droplets onto a target substrate 30, a mechanical pump device 40 being coupled with the droplet dispenser device 10 for loading a dilution liquid into the droplet dispenser device 10 and for aspirating a first portion of the suspension sample into the droplet dispenser device 10, and a syphon pump device 50 being coupled with the droplet dispenser device 10 and being arranged for aspirating a second portion of the suspension sample into the droplet dispenser device 10. Preferably, the droplet dispenser device 10 is configured for dispensing single particle droplets on the target substrate 30. Furthermore, a method of isolating particles from a suspension sample is described.