Abstract: Disclosed are thallium-sensitive assay methods and compositions for identifying effectors of monovalent ion channels, transporters, or channel-linked receptors. Further described are methods that extend the detection window of an assay to ensure assay compatibility with basic fluorescence plate readers, fluorescence microscopes, high content screening instrumentation, and flow cytometers. The ability to conduct ion mobilization screens on additional instrumentation also enables significantly more complex data acquisition, analysis, and interpretation.

Abstract: A cellularized patch for treating a scar or skin condition of a subject The cellularized patch and methods of use thereof are advantageous in skin graft procedures performed, for example, to treat a subject having a skin condition comprising skin hypopigmentation or depigmentation, such as vitiligo. In some cases, human melanocytes are cultured ex vivo and seeded onto a transfer patch before being applied to a subject.