Abstract: The present disclosure encompasses engineered meganucleases that bind and cleave recognition sequences within a dystrophin gene. The present disclosure also encompasses methods of using such engineered meganucleases to make genetically modified cells. Further, the disclosure encompasses pharmaceutical compositions comprising engineered meganuclease proteins, or polynucleotides encoding engineered meganucleases of the disclosure, and the use of such compositions for the modification of a dystrophin gene in a subject, or for treatment of Duchenne Muscular Dystrophy.

Abstract: The present invention encompasses engineered nucleases which recognize and cleave a recognition sequence within the int22h-1 sequence of a Factor VIII gene. The present invention also encompasses methods of using such engineered nucleases to make genetically-modified cells, and the use of such cells in a pharmaceutical composition and in methods for treating hemophilia A. Further, the invention encompasses pharmaceutical compositions comprising engineered nuclease proteins, nucleic acids encoding engineered nucleases, or genetically-modified cells of the invention, and the use of such compositions for treating of hemophilia A.

Abstract: A container for storing items in an insulated environment, comprising: a base having integrally formed opposing end supports projecting above a surface of the base and at least one elevated edge extending between the opposing end supports; a pair of end walls, each end wall hingedly mounted to an upper end of the end supports; a pair of side walls, one of the side walls being hingedly mounted to a surface of the base along an edge thereof to extend between the end supports and the other side wall being hingedly mounted to an upper end of the elevated edge of the base to extend between the end supports; and a pair of lid members, each lid member being hingedly mounted to an upper end of the end walls or side walls; wherein, in a first folded state each of the side walls, end walls and lid members are foldable to extend over the base in a stacked manner, and in a second assembled state each of the side walls, end walls and lid members are interengage to form a sealed and insulated storage space.

Abstract: The specification discloses a wheel carriage assembly (10) for supporting sliding doors or other similar panels with the wheel carriage assembly (10) rolling along a fixed guide track (11), the wheel carriage assembly (10) having at least one and often multiple pairs (17, 18) of laterally spaced wheels (19, 20) adapted to roll along the fixed guide track (11), the wheels (19, 20) of the or each said pair of wheels (17, 18) being mounted to a shaft (21) and at least one of the wheels (19, 20) being capable of limited axial movement relative to the shaft (21).

Abstract: The present specification discloses a carriage body (10) and a bearing (24) as a sub assembly for use in a roller wheel assembly (32), the bearing (24) centrally supporting a roller wheel supporting shaft (27) for rotation, in use, about a rotation axis defined by the rotation shaft (27), the bearing (24) having an outer peripheral mounting surface (50), the carriage body (10) being in one piece defining a bearing support cavity (17) providing a support surface (23) engaging at least a portion of the outer peripheral mounting surface (50) of the bearing (24), the one piece carriage body (10) further having a bearing access opening (28) whereby the bearing (24) is operationally positioned in the bearing support cavity (17) by movement through the access opening (28) in a direction transverse to the rotation axis defined by the support shaft (27).

Abstract: The present specification discloses a carriage body (10) and a bearing (24) as a sub assembly for use in a roller wheel assembly (32), the bearing (24) centrally supporting a roller wheel supporting shaft (27) for rotation, in use, about a rotation axis defined by the rotation shaft (27), the bearing (24) having an outer peripheral mounting surface (50), the carriage body (10) being in one piece defining a bearing support cavity (17) providing a support surface (23) engaging at least a portion of the outer peripheral mounting surface (50) of the bearing (24), the one piece carriage body (10) further having a bearing access opening (28) whereby the bearing (24) is operationally positioned in the bearing support cavity (17) by movement through the access opening (28) in a direction transverse to the rotation axis defined by the support shaft (27).

Abstract: The specification discloses a wheel carriage assembly (10) for supporting sliding doors or other similar panels with the wheel carriage assembly (10) rolling along a fixed guide track (11), the wheel carriage assembly (10) having at least one and often multiple pairs (17, 18) of laterally spaced wheels (19, 20) adapted to roll along the fixed guide track (11), the wheels (19, 20) of the or each said pair of wheels (17, 18) being mounted to a shaft (21) and at least one of the wheels (19, 20) being capable of limited axial movement relative to the shaft (21).

Abstract: A roller support assembly is used to support sliding doors, windows or the like. The assembly includes a housing member having spaced apart side walls and interconnecting transverse walls defining a roller receiving zone for receiving and operationally mounting a roller assembly. The roller assembly has a roller body with a rolling peripheral rim and a shaft whereby the roller body is rotatable relative to the shaft having short stub axle parts. The housing side walls including openings that are fully enclosed by the side walls into which the short stub axle parts fit. The housing side walls further include projecting elements extending inwardly from an inner surface of a housing side wall to cooperate with the corresponding roller body side wall surfaces to spay the housing side walls apart as the roller assembly is moved into the roller receiving zone to locate the stub axle parts in the openings.

Abstract: A roller support assembly is used to support sliding doors, windows or the like. The assembly includes a housing member having spaced apart side walls and interconnecting transverse walls defining a roller receiving zone for receiving and operationally mounting a roller assembly. The roller assembly has a roller body with a rolling peripheral rim and a shaft whereby the roller body is rotatable relative to the shaft having short stub axle parts. The housing side walls including openings that are fully enclosed by the side walls into which the short stub axle parts fit. The housing side walls further include projecting elements extending inwardly from an inner surface of a housing side wall to cooperate with the corresponding roller body side wall surfaces to spay the housing side walls apart as the roller assembly is moved into the roller receiving zone to locate the stub axle parts in the openings.