Abstract: Disclosed herein are recombinant meganucleases engineered to bind and cleave a recognition sequence in the mitochondrial DNA (mtDNA) of a eukaryotic cell, such as a plant cell. The disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been having modified or edited.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: The present invention encompasses engineered meganucleases which recognize and cleave a recognition sequence within the human PCSK9 gene. The present invention also encompasses methods for using such engineered meganucleases in a pharmaceutical composition and in methods for treating or reducing the symptoms of cholesterol-related disorders, such as hypercholesterolemia. Further, the invention encompasses pharmaceutical compositions comprising engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, and the use of such compositions for treating cholesterol-related disorders, such as hypercholesterolemia.

Abstract: The present invention encompasses engineered meganucleases which recognize and cleave a recognition sequence within the human PCSK9 gene. The present invention also encompasses methods for using such engineered meganucleases in a pharmaceutical composition and in methods for treating or reducing the symptoms of cholesterol-related disorders, such as hypercholesterolemia. Further, the invention encompasses pharmaceutical compositions comprising engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, and the use of such compositions for treating cholesterol-related disorders, such as hypercholesterolemia.

Abstract: The specification discloses a wheel carriage assembly (10) for supporting sliding doors or other similar panels with the wheel carriage assembly (10) rolling along a fixed guide track (11), the wheel carriage assembly (10) having at least one and often multiple pairs (17, 18) of laterally spaced wheels (19, 20) adapted to roll along the fixed guide track (11), the wheels (19, 20) of the or each said pair of wheels (17, 18) being mounted to a shaft (21) and at least one of the wheels (19, 20) being capable of limited axial movement relative to the shaft (21).

Abstract: The present specification discloses a carriage body (10) and a bearing (24) as a sub assembly for use in a roller wheel assembly (32), the bearing (24) centrally supporting a roller wheel supporting shaft (27) for rotation, in use, about a rotation axis defined by the rotation shaft (27), the bearing (24) having an outer peripheral mounting surface (50), the carriage body (10) being in one piece defining a bearing support cavity (17) providing a support surface (23) engaging at least a portion of the outer peripheral mounting surface (50) of the bearing (24), the one piece carriage body (10) further having a bearing access opening (28) whereby the bearing (24) is operationally positioned in the bearing support cavity (17) by movement through the access opening (28) in a direction transverse to the rotation axis defined by the support shaft (27).

Abstract: The present specification discloses a carriage body (10) and a bearing (24) as a sub assembly for use in a roller wheel assembly (32), the bearing (24) centrally supporting a roller wheel supporting shaft (27) for rotation, in use, about a rotation axis defined by the rotation shaft (27), the bearing (24) having an outer peripheral mounting surface (50), the carriage body (10) being in one piece defining a bearing support cavity (17) providing a support surface (23) engaging at least a portion of the outer peripheral mounting surface (50) of the bearing (24), the one piece carriage body (10) further having a bearing access opening (28) whereby the bearing (24) is operationally positioned in the bearing support cavity (17) by movement through the access opening (28) in a direction transverse to the rotation axis defined by the support shaft (27).

Abstract: The specification discloses a wheel carriage assembly (10) for supporting sliding doors or other similar panels with the wheel carriage assembly (10) rolling along a fixed guide track (11), the wheel carriage assembly (10) having at least one and often multiple pairs (17, 18) of laterally spaced wheels (19, 20) adapted to roll along the fixed guide track (11), the wheels (19, 20) of the or each said pair of wheels (17, 18) being mounted to a shaft (21) and at least one of the wheels (19, 20) being capable of limited axial movement relative to the shaft (21).

Abstract: A roller support assembly is used to support sliding doors, windows or the like. The assembly includes a housing member having spaced apart side walls and interconnecting transverse walls defining a roller receiving zone for receiving and operationally mounting a roller assembly. The roller assembly has a roller body with a rolling peripheral rim and a shaft whereby the roller body is rotatable relative to the shaft having short stub axle parts. The housing side walls including openings that are fully enclosed by the side walls into which the short stub axle parts fit. The housing side walls further include projecting elements extending inwardly from an inner surface of a housing side wall to cooperate with the corresponding roller body side wall surfaces to spay the housing side walls apart as the roller assembly is moved into the roller receiving zone to locate the stub axle parts in the openings.

Abstract: A roller support assembly is used to support sliding doors, windows or the like. The assembly includes a housing member having spaced apart side walls and interconnecting transverse walls defining a roller receiving zone for receiving and operationally mounting a roller assembly. The roller assembly has a roller body with a rolling peripheral rim and a shaft whereby the roller body is rotatable relative to the shaft having short stub axle parts. The housing side walls including openings that are fully enclosed by the side walls into which the short stub axle parts fit. The housing side walls further include projecting elements extending inwardly from an inner surface of a housing side wall to cooperate with the corresponding roller body side wall surfaces to spay the housing side walls apart as the roller assembly is moved into the roller receiving zone to locate the stub axle parts in the openings.