Abstract: Cell specific replication-competent viral vectors comprising a self processing peptide cleavage sequence are provided. The targeted replication-competent viral vectors include two or more co-transcribed genes under transcriptional control of the same heterologous transcriptional regulatory element (TRE), wherein at least a second gene is under translational control of a self processing cleavage sequence or 2A sequence. Exemplary vector constructs may further include an additional proteolytic cleavage site which provides a means to remove the self processing peptide sequence from the viral vector.

Abstract: PIN1 transcriptional regulatory sequences (TREs) and vectors comprising the same are provided. These include replication competent vectors and replication incompetent vectors. PIN1 TREs provide for transcriptional regulation dependent upon transcription factors that are specifically active in cancer cells. The PIN1 TREs may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity and for selective expression in cancer cells.

Abstract: Apparatus and methods for employing electrical properties of water to indicate the level of filtered water in a filtered water container. The container is fitted with a hopper that holds unfiltered water. The hopper is fitted at its base with a removable and replaceable filter cartridge. Water is filtered by draining through the filter medium contained in the filter cartridge into the lower portion of the container. The water level in the lower portion of the container is monitored by means of one or more water level detector strips in the lower portion of the container. The detector strips are in electrical communication with a detection circuit and a control unit. The control unit uses the detection strips to monitor water level and uses such data to track filtered water consumption and to determine when the filter cartridge should be replaced.

Abstract: Apparatus and methods for employing electrical properties of water to indicate the level of filtered water in a filtered water container. The container is fitted with a hopper that holds unfiltered water. The hopper is fitted at its base with a removable and replaceable filter cartridge. Water is filtered by draining through the filter medium contained in the filter cartridge into the lower portion of the container. The water level in the filtered water container is monitored by means of one or more water level detector strips in the hopper or in the lower portion of the filtered water container. The detector strips are in electrical communication with a detection circuit and a control unit. The control unit uses the detection strips to monitor water level and uses such data to track filtered water consumption and to determine when the filter cartridge should be replaced.

Abstract: Cell specific replication-competent viral vectors comprising a self processing peptide cleavage sequence are provided. The targeted replication-competent viral vectors include two or more co-transcribed genes under transcriptional control of the same heterologous transcriptional regulatory element (TRE), wherein at least a second gene is under translational control of a self processing cleavage sequence or 2A sequence. Exemplary vector constructs may further include an additional proteolytic cleavage site which provides a means to remove the self processing peptide sequence from the viral vector.