Abstract: An acoustic wave biosensor comprises a plurality of spaced apart electrodes disposed on a substrate of piezoelectric material and having a biolayer matched to a specific type of autoinducer signaling molecule to be detected. The biolayer comprises a layer of heterobifunctional molecules disposed on the electrodes and on the piezoelectric material between the electrodes, and a plurality of bioreceptor molecules which bind exclusively with the specific type of autoinducer signaling molecule to be detected. The bioreceptor molecules are supported by the layer of heterobifunctional molecules, and a hydrogel layer surrounds the bioreceptor molecules to support a three-dimensional structure thereof. The bioreceptor molecules can bind with the specific type of autoinducer signaling molecule to be detected, and the biolayer is reactive thereto such that corresponding autoinducer signaling molecules bind to the biolayer and detectably vary acoustic characteristics of the acoustic wave biosensor.

Abstract: An acoustic wave biosensor comprises a plurality of spaced apart electrodes disposed on a substrate of piezoelectric material and having a biolayer matched to a specific type of autoinducer signaling molecule to be detected. The biolayer comprises a layer of heterobifunctional molecules disposed on the electrodes and on the piezoelectric material between the electrodes, and a plurality of bioreceptor molecules which bind exclusively with the specific type of autoinducer signaling molecule to be detected. The bioreceptor molecules are supported by the layer of heterobifunctional molecules, and a hydrogel layer surrounds the bioreceptor molecules to support a three-dimensional structure thereof. The bioreceptor molecules can bind with the specific type of autoinducer signaling molecule to be detected, and the biolayer is reactive thereto such that corresponding autoinducer signaling molecules bind to the biolayer and detectably vary acoustic characteristics of the acoustic wave biosensor.

Abstract: A method of detecting and identifying bacteria, micro-organisms or plants in a liquid or gaseous medium, the bacteria, micro-organisms or plants being of the kind which produce signaling molecules in intercellular space, includes positioning a biosensor in the liquid or gaseous medium, the biosensor having a biolayer matched to specific signaling molecules to be detected whereby the biolayer is reactive thereto in a manner which varies operation of the sensor. Such variation of the operation of the biosensor is detected to thereby determine the presence and purpose of the bacteria, micro-organisms or plants in the liquid or gaseous medium.

Abstract: A biosensor detection system for detecting a particular substance has at least two biosensor devices. Each biosensor device includes a piezoelectric material, an input transducer mounted on the piezoelectric material to receive an input radio frequency signal and propagate a corresponding acoustic wave within the piezoelectric material and an output transducer mounted on the piezoelectric material to receive the acoustic wave and transmit a corresponding output radio frequency signal. A biolayer is mounted on the piezoelectric material to receive a substance to be tested and cause a corresponding change in the acoustic wave. An oscillator circuit is connected to the input transducer and to the output transducer, the oscillator circuit including an amplifier and providing an output signal indicative of a change in the acoustic wave.

Abstract: A method of detecting and identifying bacteria, micro-organisms or plants in a liquid or gaseous medium, the bacteria, micro-organisms or plants being of the kind which produce signaling molecules in intercellular space. The method includes positioning a biosensor in the liquid or gaseous medium, the biosensor having a biolayer matched to specific signaling molecules to be detected whereby the biolayer is reactive thereto in a manner which varies operation of the sensor. Such variation of the operation of the biosensor is detected to thereby determine the presence and purpose of the bacteria, micro-organisms or plants in the liquid or gaseous medium.

Abstract: A deployable acoustic wave RFID/biosensor assembly has at least one protective layer, and an acoustic wave RFID/biosensor within the protective layer or layers. The acoustic wave RFID/biosensor includes an acoustic wave device, a biolayer mounted on the acoustic wave device, an electric circuit operable to actuate the acoustic wave device, and a fluidic chamber associated with the biolayer. In use, the fluidic chamber contains fluid which, if a predetermined substance to be sensed is present, operates to modify the biolayer which in turn affects the operation of the acoustic wave device. Protective fluid is provided within the protective layer or layers substantially surrounds the acoustic wave RFID/biosensor to protect the acoustic wave RFID/biosensor from damage when the RFID/biosensor assembly impacts a target and to protect the biolayer from deterioration during storage and use.