Abstract: The present application provides TILs comprising agents that enhance activity and/or proliferative capacity of the TILs, methods of manufacturing such TILs. and methods of using such modified TILs for enhancing an immune response.

Abstract: The present disclosure provides methods for reprogramming a cell, wherein the method comprises passing a cell suspension comprising the cell and a reprogramming factor through a constriction, wherein the constriction deforms the cell, thereby causing a perturbation of the cell such that the reprogramming factor enters the cell.

Abstract: The present invention provides methods for stimulating an immune response to an antigen comprising administering to an individual, an anucleate cell-derived vesicle comprising an antigen and/or an adjuvant. In some embodiments, the anucleate cell-derived vesicle comprising the antigen and/or adjuvant is generated by passing a cell suspension containing an input anucleate cell through a constriction, wherein the constriction deforms the input anucleate cell thereby causing a perturbation of the cell to form an anucleate cell-derived vesicle such that an antigen and/or an adjuvant enters the anucleate cell-derived vesicle. In some embodiments, the anucleate cell-derived vesicle comprising the antigen and/or adjuvant is delivered to an individual and the antigen is delivered to and processed in an immunogenic environment to treat a disease, prevent a disease, and/or vaccinate an individual against an antigen.

Abstract: A tissue measurement system includes a clamp, a measuring head, and a deflection sensor. The clamp includes a first plate and a second plate releasably engageable with one another to secure a substantially planar biological tissue. In the engaged position, the first and second plates define at least a portion of a test section along which a portion of the secured substantially planar biological tissue is disposed. The measuring head is movable toward the test section and into contact with the portion of the substantially planar biological tissue disposed along the test section. The deflection sensor is responsive to movement of the biological tissue contacted by the measuring head.

Abstract: A tissue measurement system includes a clamp, a measuring head, and a deflection sensor. The clamp includes a first plate and a second plate releasably engageable with one another to secure a substantially planar biological tissue. In the engaged position, the first and second plates define at least a portion of a test section along which a portion of the secured substantially planar biological tissue is disposed. The measuring head is movable toward the test section and into contact with the portion of the substantially planar biological tissue disposed along the test section. The deflection sensor is responsive to movement of the biological tissue contacted by the measuring head.