Abstract: Methods for characterizing one or more molecules based upon detection of time-dependent changes in binding interactions between the molecules and binding entities are provided. Characterizations of individual molecules by provided methods include identification of the molecules and determination of previously uncharacterized time-dependent binding interactions between the molecules and binding entities.

Abstract: The present disclosure provides methods of determining association rates or dissociation rates between affinity reagents and proteins. The methods can be configured to monitor a large number of proteins in parallel, for example, using arrays of proteins that are contacted with solutions containing affinity reagents. The methods can be further configured to detect the arrayed proteins at single-molecule resolution. Accordingly the methods allow a large population of proteins to be monitored on an individual basis. As such binding kinetics and thermodynamics can be determined on a population level while allowing individual interactions to be evaluated.

Abstract: The present disclosure relates to methods and systems for identifying polypeptides using nucleic acid data obtained from a polypeptide sequencing device that encode amino acid sequence information into barcoded nucleic acid sequences that are later analyzed in a nucleic acid sequencer. Nucleic acid data generated as an output of the nucleic acid sequencer are processed to retrieve information about the analyzed polypeptides. The process includes generating a plurality of binder identifier strings for the generated nucleic acid data, and providing the plurality of binder identifier strings as input to a computer model configured to infer amino acid sequences of polypeptides from binder identifier strings. The disclosure finds utility at least in a variety of methods and related systems for high-throughput polypeptide analysis and sequencing.

Abstract: The present disclosure relates to methods for high throughput analysis of analytes such as polypeptides in a cyclic manner (e.g., using NGPA or NGPS described herein) that allow adjustment of the dynamic range and sensitivity of analyte detection, for instance, based on the abundances of different polypeptides present in a sample to be analyzed. The approaches proposed herein can be used to adjust the dynamic range of abundant analytes (e.g., polypeptides present in high concentrations) in biological samples, such as plasma samples, and to increase proteome coverage achieved by high throughput protein analysis methods, for instance, by improving the sensitivity of detecting less abundant analytes.

Abstract: A fiber Bragg grating sensor arrangement includes a resonant cavity light emitting diode for outputting light; a fiber having a first end disposed to receive light output from the resonant cavity light emitting diode, the fiber including fiber Bragg grating etched at one or more locations along a length thereof. A strain mount or beam supports the fiber and to which the fiber is attached or bonded. A light detection circuit disposed at a second end of the fiber receives light traveling through the fiber, the light detection circuit sensing intensity of the received light that corresponds to strain or force applied to the fiber that is bonded to the strain mount. Another fiber Bragg grating sensor arrangement includes a second reference fiber that does not receive a force or strain. The reference fiber provides an output used to prevent temperature/humidity from affecting the output of the FBG sensor arrangement.

Abstract: Provided herein are high-throughput, population-wide serotyping and antibody profiling assays. Disclosed variants of a Digital Serotyping assay employ next generation sequencing to measure the “serotyping profile” of barcoded subject serum antibodies tested against a range of DNA-tagged pathogen-derived antigens. The disclosed assay setup enables multiplexing in both the sample and antigen dimensions, generating a large multi-dimensional serotyping data set for more comprehensive serotyping profiling of large populations across a large number of antigens and possible pathogens. Moreover, the ability to easily scale and multiplex the number of peptide epitopes allows rapid updating of the assay content to monitor the ever-changing spectrum of pathogens. Additional applications of this technology include cancer immunology and autoimmune conditions (e.g., neoantigen or autoimmune profiling), screening for toxins, antibody therapeutics development, biosecurity, and veterinary medicine.

Abstract: Provided herein are high-throughput, population-wide serotyping and antibody profiling assays. Disclosed variants of a Digital Serotyping assay employ next generation sequencing to measure the “serotyping profile” of barcoded subject serum antibodies tested against a range of DNA-tagged pathogen-derived antigens. The disclosed assay setup enables multiplexing in both the sample and antigen dimensions, generating a large multi-dimensional serotyping data set for more comprehensive serotyping profiling of large populations across a large number of antigens and possible pathogens. Moreover, the ability to easily scale and multiplex the number of peptide epitopes allows rapid updating of the assay content to monitor the ever-changing spectrum of pathogens. Additional applications of this technology include cancer immunology and autoimmune conditions (e.g., neoantigen or autoimmune profiling), screening for toxins, antibody therapeutics development, biosecurity, and veterinary medicine.

Abstract: Provided herein are high-throughput, population-wide serotyping and antibody profiling assays. Disclosed variants of a Digital Serotyping assay employ next generation sequencing to measure the “serotyping profile” of barcoded subject serum antibodies tested against a range of DNA-tagged pathogen-derived antigens. The disclosed assay setup enables multiplexing in both the sample and antigen dimensions, generating a large multi-dimensional serotyping data set for more comprehensive serotyping profiling of large populations across a large number of antigens and possible pathogens. Moreover, the ability to easily scale and multiplex the number of peptide epitopes allows rapid updating of the assay content to monitor the ever-changing spectrum of pathogens. Additional applications of this technology include cancer immunology and autoimmune conditions (e.g., neoantigen or autoimmune profiling), screening for toxins, antibody therapeutics development, biosecurity, and veterinary medicine.