Patents by Inventor Dhruv Sareen
Dhruv Sareen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200224159Abstract: A unified cell differentiation protocol for obtaining photoreceptor cells, retinal pigment epithelium, and 3D retinal organoid from pluripotent stem cells is described. Also described are photoreceptor cells, retinal pigmented epithelium, and 3D retinal organoid obtained from pluripotent stem cells. Also described are a pharmaceutical composition and a medicament containing the photoreceptor cells, retinal pigment epithelium, and 3D retinal organoid as described.Type: ApplicationFiled: October 5, 2018Publication date: July 16, 2020Inventors: Rajarshi PAL, Rajani BATTU, Reena RATHOD, Harshini SURENDRAN, Kapil BHARTI, Deepak LAMBA, Dhruv SAREEN, Mahendra RAO, Sushma NANJUNDA SWAMY, Vijay Bhaskar KONALA REDDY, Mohanapriya RAJAMOORTHY
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Publication number: 20200157508Abstract: Induced pluripotent stem cell (iPSC)-based organoid technology has tremendous potential to elucidate the intestinal and colonic epithelium's role in health and disease. Described herein are methods and compositions for generation of intestinal and colonic cells from iPSCs. Derivation of iPSCs from subjected afflicted with early onset and very early onset Inflammatory Bowel Disease (IBD), serves as an excellent model for understanding disease pathogenesis.Type: ApplicationFiled: May 18, 2018Publication date: May 21, 2020Applicant: Cedares-Sinai Medical CenterInventors: Robert Barrett, Clive Svendsen, Stephan R. Targan, Michael Workman, Dhruv Sareen
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Publication number: 20200071673Abstract: The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).Type: ApplicationFiled: March 14, 2018Publication date: March 5, 2020Applicant: CEDARS-SINAI MEDICAL CENTERInventors: Dhruv SAREEN, Berhan MANDEFRO, Anjoscha KAUS
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Publication number: 20200002671Abstract: Human induced pluripotent stem cells (iPSCs) can give rise to multiple cell types and hold great promise in regenerative medicine and disease modeling applications. The Inventors herein developed a reliable two-step protocol to generate human mammary-like organoids from iPSCs. Non-neural ectoderm cell-containing spheres, referred to as mEBs, were first differentiated and enriched from iPSCs using MammoCult medium. Gene expression profile analysis suggested that mammary gland function-associated signaling pathways were hallmarks of 10-d differentiated mEBs. The Inventors generated mammary-like organoids from 10-d mEBs using 3D floating mixed gel culture and a three-stage differentiation procedure. These organoids expressed common breast tissue, luminal, and basal markers, including estrogen receptor, and could be induced to produce milk protein. These results demonstrate that human iPSCs can be directed in vitro toward mammary lineage differentiation.Type: ApplicationFiled: January 25, 2018Publication date: January 2, 2020Applicant: Cedars-Sinai Medical CenterInventors: Ying QU, Xiaojiang CUI, Dhruv SAREEN, Armando E. GIULIANO
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Publication number: 20190359924Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.Type: ApplicationFiled: February 26, 2019Publication date: November 28, 2019Inventors: S. Jordan Kerns, Norman Wen, Carolina Lucchesi, Christopher David Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Samuel Sances, Clive Svendsen, Daniel Levner, Dhruv Sareen
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Publication number: 20190194606Abstract: Described here are systems and methods for deriving both spinal motor neurons and brain microvascular endothelial cells from induced pluripotent stem cells using distinct methods and combining them in a chip format. Neurons cultured alone in chip microvolume displayed increased calcium transient function and chip-specific gene expression. When seeded with endothelial cells, interaction further enhanced neural function, elicited vascular-neural interaction, niche gene expression with enhanced in vivo-like signatures arising from the chip co-cultures. Development of novel media formulations further allow for improved readout of differentiation process, by eliminating additives that otherwise confound differentiation processes and resulting phenotypes.Type: ApplicationFiled: August 29, 2017Publication date: June 27, 2019Applicant: CEDARS-SINAI MEDICAL CENTERInventors: Gad VATINE, Sam SANCES, Clive SVENDSEN, Dhruv SAREEN, Alexis J. KERL
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Publication number: 20190194624Abstract: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.Type: ApplicationFiled: June 16, 2017Publication date: June 27, 2019Applicant: Cedars-Sinai Medical CenterInventors: Dhruv SAREEN, Loren A. ORNELAS, Clive SVENDSEN
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Publication number: 20190153395Abstract: Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.Type: ApplicationFiled: February 1, 2017Publication date: May 23, 2019Applicant: Cedars-Sinai Medical CenterInventors: Robert Barrett, Clive Svendsen, Stephan R. Targan, Michael Workman, Dhruv Sareen, Uthra Rajamani
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Publication number: 20190136203Abstract: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.Type: ApplicationFiled: January 14, 2019Publication date: May 9, 2019Applicant: CEDARS-SINAI MEDICAL CENTERInventors: Dhruv Sareen, Loren A. Ornelas, Clive Svendsen
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Patent number: 10221395Abstract: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.Type: GrantFiled: June 16, 2016Date of Patent: March 5, 2019Assignee: Cedars-Sinai Medical CenterInventors: Dhruv Sareen, Loren A. Ornelas, Clive Svendsen
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Publication number: 20190031992Abstract: Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.Type: ApplicationFiled: July 31, 2018Publication date: January 31, 2019Inventors: S. Jordan Kerns, Norman Wen, Carol Lucchesi, Christopher David Hinojosa, Jacob Fraser, Jefferson Puerta, Geraldine Hamilton, Robert Barrett, Clive Svendsen, Daniel Levner, Stephen R. Targan, Michael Workman, Dhruv Sareen, Uthra Rajamani, Magdalena Kasendra
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Publication number: 20180305651Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.Type: ApplicationFiled: October 19, 2016Publication date: October 25, 2018Inventors: S. Jordan Kerns, Norman Wen, Carolina Lucchesi, Christopher David Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Samuel Sances, Clive Svendsen, Daniel Levner, Dhruv Sareen
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Publication number: 20180298332Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.Type: ApplicationFiled: April 17, 2018Publication date: October 18, 2018Inventors: S. Jordan Kerns, Norman Wen, Carolina Lucchesi, Christopher David Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Samuel Sances, Clive Svendsen, Daniel Levner, Dhruv Sareen
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Publication number: 20180298331Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.Type: ApplicationFiled: April 17, 2018Publication date: October 18, 2018Inventors: S. Jordan Kerns, Norman Wen, Carolina Lucchesi, Christopher David Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Samuel Sances, Clive Svendsen, Daniel Levner, Dhruv Sareen
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Publication number: 20180057788Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.Type: ApplicationFiled: November 15, 2016Publication date: March 1, 2018Inventors: Jordan Kerns, Norman Wen, Carol Lucchesi, Christopher Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Sam Sances, Clive Svendsen, Daniel Levner, Dhruv Sareen
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Publication number: 20170362574Abstract: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.Type: ApplicationFiled: June 16, 2016Publication date: December 21, 2017Applicant: Cedars-Sinai Medical CenterInventors: Dhruv Sareen, Loren A. Ornelas, Clive Svendsen
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Publication number: 20170226478Abstract: The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).Type: ApplicationFiled: March 14, 2017Publication date: August 10, 2017Inventors: Jordan Kerns, Norman Wen, Geraldine Hamilton, Christopher Hinojosa, Jacob Fraser, Catherine Karalis, Janna Nawroth, Dhruv Sareen, Anjoscha Kaus, Berhan Mandefro, Hyoung Shin Park, Ville Kujala
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Publication number: 20170107498Abstract: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors, including generation of iPSCs from lymphoblastoid B-cells and lymphoblastoid B-cell lines. Such methods and compositions find use in regenerative medicine applications.Type: ApplicationFiled: June 5, 2015Publication date: April 20, 2017Applicant: Cedars-Sinai Medical CenterInventors: Dhruv Sareen, Loren Ornelas, Robert Barrett