Patents by Inventor Diane G. Holmstrom

Diane G. Holmstrom has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of multiplying conifer embryogenic tissue
    Patent number: 8999713
    Abstract: The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones; and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).
    Type: Grant
    Filed: September 4, 2012
    Date of Patent: April 7, 2015
    Assignee: Weyerhaeuser NR Company
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Pamela L. Carlson
  • METHODS OF MULTIPLYING CONIFER EMBRYOGENIC TISSUE
    Publication number: 20130078722
    Abstract: The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones; and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).
    Type: Application
    Filed: September 4, 2012
    Publication date: March 28, 2013
    Applicant: WEYERHAEUSER NR COMPANY
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Pamela L. Carlson
  • Development and stratification of pine somatic embryos using a liquid system
    Patent number: 7732205
    Abstract: The invention provides methods for producing pine somatic embryos using a liquid development medium and/or a liquid stratification medium. In a first aspect, the methods comprise the step of culturing embryogenic cells in, or on, a liquid development medium to produce cotyledonary pine somatic embryos. In another aspect, the methods comprise the step of culturing pine cotyledonary somatic embryos in, or on, a liquid stratification medium to produce stratified cotyledonary somatic embryos. The invention also provides methods for producing pine somatic embryos in bioreactors.
    Type: Grant
    Filed: June 24, 2004
    Date of Patent: June 8, 2010
    Assignee: Weyerhaeuser NR Company
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Bonnie Larson, Judith Zucati
  • Continuous culture of conifer embryogenic tissue
    Patent number: 7625754
    Abstract: The present invention provides methods for multiplying conifer embryogenic tissue. The methods of the present invention each include the step of continuously culturing conifer embryogenic tissue in liquid multiplication medium for a period of time sufficient for the embryogenic tissue to multiply.
    Type: Grant
    Filed: February 22, 2005
    Date of Patent: December 1, 2009
    Assignee: Weyerhaeuser NR Company
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Bonnie Larson
  • Methods for developing conifer somatic embryos
    Patent number: 7452722
    Abstract: The present invention provides methods for producing conifer cotyledonary somatic embryos. The methods of the present invention each includes the step of culturing embryogenic conifer tissue in, or on, a development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos from the embryogenic conifer tissue, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
    Type: Grant
    Filed: February 7, 2005
    Date of Patent: November 18, 2008
    Assignee: Weyerhaeuser Company
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Bonnie Larson
  • Embryogenic culture initiation of douglas-fir by maltose
    Publication number: 20040237130
    Abstract: In one aspect, the present invention provides methods for producing Douglas-fir somatic embryos. In one aspect, the methods of the invention comprise the step of culturing Douglas-fir explants in, or on, an initiation medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form embryonal suspensor masses comprising early-stage somatic embryos. In another aspect, the methods of the invention additionally comprise the step of culturing Douglas-fir embryonal suspensor masses in maintenance medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form an established embryonal suspensor mass comprising early-stage somatic embryos.
    Type: Application
    Filed: April 7, 2004
    Publication date: November 25, 2004
    Applicant: Weyerhaeuser Company
    Inventors: Pramod K. Gupta, Diane G. Holmstrom, Doris Budworth