Abstract: Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.

Abstract: Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.

Abstract: Methods and compositions for performing template dependent nucleic acid primer extension reactions that produce a reduced complexity product are provided. In the subject methods, template RNA is contacted with a primer composition that includes both a universal, e.g., oligo dT, primer and at least one gene specific primer under template dependent primer extension reaction conditions, which step results in the production of a reduced complexity product as compared to the initial RNA template. The subject methods find use a variety of different applications, including the preparation of labeled nucleic acids, e.g., for use in differential gene expression analysis applications. Also provided are kits for practicing the subject methods.