Patents by Inventor Diane R. Bienek

Diane R. Bienek has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization
    Patent number: 7504202
    Abstract: The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.
    Type: Grant
    Filed: March 26, 2004
    Date of Patent: March 17, 2009
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Malford E. Cullum, Paul Hine, Lloyd G. Simonson, Chun N. Shih, Diane R. Bienek, Sukjoon Park, James C. Ragain, Jr., Linda A. Lininger
  • Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization
    Publication number: 20040235075
    Abstract: The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.
    Type: Application
    Filed: March 26, 2004
    Publication date: November 25, 2004
    Inventors: Malford E. Cullum, Paul Hine, Lloyd G. Simonson, Chun N. Shih, Diane R. Bienek, Sukjoon Park, James C. Ragain