Patents by Inventor Diane Retallack
Diane Retallack has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170183673Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.Type: ApplicationFiled: March 8, 2017Publication date: June 29, 2017Inventors: Donald J. Merlo, Sean M. Russell, Diane Retallack, Aaron Woosley, Tom Meade, Kenneth E. Narva
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Publication number: 20110111977Abstract: Methods of identifying and expressing an antibody variant are disclosed wherein the method comprises identifying a binding region in an antibody, fusing the binding region to a plurality of scaffolds of antibody constant regions to obtain antibody fragment variants, expressing the antibody fragment variants in organisms to form constructs and expressing the constructs carried by the organisms to form induced cultures, wherein the organisms are expressed in HTP mode.Type: ApplicationFiled: July 1, 2009Publication date: May 12, 2011Applicant: Pfenex, Inc.Inventor: Diane Retallack
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Publication number: 20110020868Abstract: Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.Type: ApplicationFiled: September 16, 2010Publication date: January 27, 2011Applicant: PFENEX, INC.Inventors: Russell J. Coleman, Diane Retallack, Stacey L. Lee, Charles D. Hershberger
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Publication number: 20100048864Abstract: Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.Type: ApplicationFiled: October 22, 2009Publication date: February 25, 2010Applicant: Dow Global Technologies Inc.Inventors: Russell J. Coleman, Diane Retallack, Charles D. Hershberger, Stacey Lee
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Patent number: 7618799Abstract: Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed.Type: GrantFiled: January 30, 2008Date of Patent: November 17, 2009Assignee: Dow Global Technologies IncInventors: Russell J. Coleman, Diane Retallack, Jane C. Schneider, Thomas M. Ramseier, Charles D. Hershberger, Stacey Lee, Sol M. Resnick
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Publication number: 20090042254Abstract: Improved expression of active antibody fragments (Fabs) is achieved by truncating a heavy chain constant region. Truncation of the CH1 domain of a Fab fragment can increase yield of soluble active antibody fragment in Pseudomonas fluorescens. Another embodiment of the invention includes secretion of the light chain and a fragment of the heavy chain with various C-termini (e.g., VH-CH1 truncated to different lengths). The truncated CH1 region can be used as a scaffold to create other Fabs. Also included is truncation of the kappa light chain and/or lambda light chain domains of a Fab fragment. The invention also includes expression of Fab fragments fused to other peptides or molecules (e.g., toxins, proteins, peptides, enzymes, etc.).Type: ApplicationFiled: June 6, 2008Publication date: February 12, 2009Applicant: DOW GLOBAL TECHNOLOGIES INC.Inventors: Diane Retallack, Jon C. Mitchell
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Publication number: 20080193974Abstract: Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed.Type: ApplicationFiled: January 30, 2008Publication date: August 14, 2008Applicant: DOW GLOBAL TECHNOLOGIES, INC.Inventors: Russell J. Coleman, Diane Retallack, Jane C. Schneider, Thomas M. Ramseier, Charles D. Hershberger, Stacey Lee, Sol M. Resnick
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Publication number: 20060234346Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: ApplicationFiled: April 7, 2006Publication date: October 19, 2006Inventors: Diane Retallack, Charles Squires, David Watkins, Stacey Lee, Frank Gaertner, Robert Shutter
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Publication number: 20060040352Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: ApplicationFiled: January 18, 2005Publication date: February 23, 2006Inventors: Diane Retallack, Charles Squires, David Watkins, Stacey Lee, Frank Gaertner, Robert Shutter
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Publication number: 20060008877Abstract: Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.Type: ApplicationFiled: November 22, 2004Publication date: January 12, 2006Applicant: Dow Global Technologies Inc.Inventors: Diane Retallack, Jane Schneider, Thomas Ramseier
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Publication number: 20050202544Abstract: Novel benzoate- or anthranilate-inducible promoters, and novel tandem promoters, and variants and improved mutants thereof, useful for commercial prokaryotic fermentation systems, nucleic acid constructs containing the promoters, expression systems using them, methods for expressing proteins by use thereof, and proteins expressed thereby.Type: ApplicationFiled: January 3, 2005Publication date: September 15, 2005Applicant: Dow Global technologies Inc.Inventors: Diane Retallack, Venkiteswaran Subramanian