Patents by Inventor Dianna Maar

Dianna Maar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240279730
    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
    Type: Application
    Filed: May 3, 2024
    Publication date: August 22, 2024
    Inventors: Duc Do, Claudia Litterst, Dianna Maar
  • Publication number: 20240253042
    Abstract: Provided herein are microfluidic devices, and related processes for making and methods of using, wherein the devices include tubes with partitioned hydrophilic and hydrophobic fluids.
    Type: Application
    Filed: February 23, 2024
    Publication date: August 1, 2024
    Inventors: Dianna Maar, Nicholas Heredia, Clinton L. Torres
  • Patent number: 12024737
    Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.
    Type: Grant
    Filed: August 6, 2021
    Date of Patent: July 2, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20240198336
    Abstract: Provided herein are microfluidic devices, and related processes for making and methods of using, wherein the devices include tubes with partitioned hydrophilic and hydrophobic fluids.
    Type: Application
    Filed: December 14, 2023
    Publication date: June 20, 2024
    Inventors: Dianna Maar, Nicholas Heredia, Clinton L. Torres
  • Patent number: 12006537
    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
    Type: Grant
    Filed: June 30, 2022
    Date of Patent: June 11, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Duc Do, Claudia Litterst, Dianna Maar
  • Publication number: 20240124927
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Application
    Filed: January 3, 2024
    Publication date: April 18, 2024
    Inventors: Nicholas HEREDIA, Dianna MAAR
  • Patent number: 11898201
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Grant
    Filed: January 13, 2021
    Date of Patent: February 13, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Nicholas Heredia, Dianna Maar
  • Publication number: 20230057343
    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
    Type: Application
    Filed: June 30, 2022
    Publication date: February 23, 2023
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Duc DO, Claudia LITTERST, Dianna MAAR
  • Publication number: 20220259680
    Abstract: Methods and compositions for characterizing a biological sample (e.g., comprising an infectious agent) from a subject are provided. Methods can include detecting linkage of nucleic acids that are linked in a viable cell or organism but that become degraded and thus unlinked in inviable cells or organisms and then characterizing the subject based on the quantity of linked and unlinked sequences.
    Type: Application
    Filed: February 14, 2022
    Publication date: August 18, 2022
    Inventors: Dianna MAAR, Monica HERRERA, George KARLIN-NEUMANN, Josh SHINOFF, Audrey AUDETAT, Scott HUTTON, Hestia MELLERT, Leisa JACKSON, Gary PESTANO
  • Patent number: 11377684
    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: July 5, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Duc Do, Claudia Litterst, Dianna Maar
  • Publication number: 20220033883
    Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.
    Type: Application
    Filed: August 6, 2021
    Publication date: February 3, 2022
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: John F. REGAN, Dianna MAAR, Dawne N. SHELTON, Tina C. LEGLER, Samantha COOPER, Wei YANG, Eli A. HEFNER, Niels KLITGORD
  • Publication number: 20210388429
    Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.
    Type: Application
    Filed: August 30, 2021
    Publication date: December 16, 2021
    Inventor: Dianna MAAR
  • Patent number: 11104938
    Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.
    Type: Grant
    Filed: June 20, 2018
    Date of Patent: August 31, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Dianna Maar
  • Patent number: 11085070
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Grant
    Filed: March 30, 2020
    Date of Patent: August 10, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Patent number: 11072820
    Abstract: Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: July 27, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Eli A. Hefner, Dianna Maar
  • Patent number: 11066698
    Abstract: Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: July 20, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Dianna Maar, Samantha Cooper, Wei Yang
  • Publication number: 20210207208
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Application
    Filed: January 13, 2021
    Publication date: July 8, 2021
    Inventors: Nicholas HEREDIA, Dianna MAAR
  • Patent number: 10920270
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Grant
    Filed: March 22, 2019
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Nicholas Heredia, Dianna Maar
  • Publication number: 20200270677
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: March 30, 2020
    Publication date: August 27, 2020
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: John F. REGAN, Dianna MAAR, Dawne N. SHELTON, Tina C. LEGLER, Samantha COOPER, Wei YANG, Eli A. HEFNER, Niels KLITGORD
  • Patent number: 10718008
    Abstract: Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: July 21, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Dianna Maar, Samantha Cooper, Wei Yang