Abstract: The disclosure pertains to a microfluidic cartridge comprising at least one microchannel and at least a set of functionalized microcarriers, the microcarriers being localized within the microchannel, wherein the functionalized microcarriers are coated with at least a lyoprotectant.

Abstract: A method for determining fluorescence values {?ispe}i?{1, 2, . . . , I} of a set of I fluorescent microparticles {?Pi}i?{1, 2, . . . , I} of a multiplexed analysis, the microparticles being in a monolayer arrangement, includes acquiring a digital fluorescence image of the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}; and computing, for each fluorescent microparticle ?Pi in the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}, a fluorescence value ?imeas based only on pixels of the acquired image corresponding to said fluorescent microparticle ?Pi. The method includes computing the fluorescence value ?ispe of the fluorescent microparticle ?Pi by correcting its first fluorescence ?imeas by a cross-talk fluorescence contribution ?icross in the first fluorescence ?imeas from other fluorescent microparticles {?Pj}j?i in the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}.

Abstract: A method for determining fluorescence values {?ispe}i?{1, 2, . . . , I} of a set of I fluorescent microparticles {?Pi}i?{1, 2, . . . , I} of a multiplexed analysis, the microparticles being in a monolayer arrangement, includes acquiring a digital fluorescence image of the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}; and computing, for each fluorescent microparticle ?Pi in the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}, a fluorescence value ?imeas based only on pixels of the acquired image corresponding to said fluorescent microparticle ?Pi. The method includes computing the fluorescence value ?ispe of the fluorescent microparticle ?Pi by correcting its first fluorescence ?imeas by a cross-talk fluorescence contribution ?icross in the first fluorescence ?imeas from other fluorescent microparticles {?Pj}j?i in the set of fluorescent microparticles {?Pi}i?{1, 2, . . . , I}.

Abstract: The present invention relates to a microcarrier comprising at least a detection surface for performing an assay, said detection surface comprising a first area being functionalized with a first functional group for detecting at least a chemical and/or biological interaction, said first area being designed for providing a first signal. The microcarrier is characterized in that the detection surface further comprises a second area being designed for providing a second signal different from the first signal, said second signal being emitted during the assay. Thus, information about the presence of the at least a chemical and/or biological interaction is provided by a comparison of the first signal and the second signal.

Abstract: The present invention relates to a composition for storing at least one biomolecule, the composition comprising: a. between about 0.2% w/w to about 20.0% w/w of D-(+)-Trehalose dehydrate; and b. between about 0.1% w/w to about 10.0% w/w of D-Mannitol; c. between about 0.01% w/w to 0.3% w/w of polyoxyethylenesorbitan monooleate; and d. between about 1.0 nM to about 500 mM of Sodium Citrate at pH between about 4.0 to about 8.0; and e. between strictly more than about 0% w/w to about 0.5% w/w of preservative solution, said preservative solution comprising: i. between about 1.0% w/w and about 5.0% w/w of 5 Chloro-2 methyl-4-isothiazolin-3-one; and ii. between about 0.1% w/w and about 3.0% w/w 2-Methyl-4-isothiazolin-3-one.

Abstract: A method for performing a chemical and/or a biological assay including the following successive steps of: a) providing an assay device with a microchannel having an inlet and an outlet and restricting means for restricting movement toward the outlet of microparticles introduced in the microchannel while letting a fluid to flow through the restricting means, b) introducing microparticles in the microchannel via the inlet, c) restricting the movement of said microparticles in the microchannel toward the outlet using the restricting means, d) flowing a fluid sample through the microchannel, and e) performing a biological and/or chemical read-out on each microparticle. The method also includes the steps of: f) moving the microparticles in the microchannel, and g) repeating successively the steps d) and e).

Abstract: The present invention relates to a method for performing a chemical and/or a biological assay comprising the following successive steps of: a) providing an assay device with a microchannel having an inlet and an outlet and further comprising restricting means designed to restrict the movement toward the outlet of microparticles introduced in the microchannel while letting a fluid to flow through the restricting means, b) introducing microparticles in the microchannel via the inlet, c) restricting the movement of said microparticles in the microchannel toward the outlet using restricting means, d) flowing a fluid sample through the microchannel, e) performing a biological and/or chemical read-out on each microparticle, the method further comprising the steps of: f) moving the microparticles in the microchannel, and g) repeating successively the steps d) and e).