Patents by Inventor Didier Lefevre
Didier Lefevre has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8080383Abstract: A subject of the invention is a method for discriminating between and counting at least two populations of biological elements carrying specific characteristics, optionally present in a sample. The invention allows the clear and unambiguous detection of at least three populations of biological elements by the use of only two detection means, which means that at least two populations of biological elements are detected by one and the same detection means. The invention can be carried out if three different probes are used, each recognizing and becoming fixed to one of the populations of biological elements to be detected, each of the probes being itself rendered detectable by a different marker, two of said markers having two emission spectra having at least one common part (overlapping emission spectra) and the third having an emission spectrum having essentially no part in common with the other two (non-overlapping spectrum).Type: GrantFiled: December 5, 2006Date of Patent: December 20, 2011Assignee: Horiba Abx Sas, Parc EuromedicineInventors: Francis Lacombe, Francis Belloc, Sylvie Veriac, Didier Lefevre
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Patent number: 8008029Abstract: The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.Type: GrantFiled: July 6, 2005Date of Patent: August 30, 2011Assignee: Horiba ABX SASInventor: Didier Lefevre
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Patent number: 7777869Abstract: The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.Type: GrantFiled: April 14, 2006Date of Patent: August 17, 2010Assignee: Horiba ABX SASInventors: Philippe Nerin, Didier Lefevre
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Patent number: 7638290Abstract: The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.Type: GrantFiled: December 29, 2006Date of Patent: December 29, 2009Assignee: ABXInventors: Didier Lefevre, Sylvie Veriac, Henri Champseix
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Publication number: 20090311678Abstract: A subject of the invention is a method for discriminating between and counting at least two populations of biological elements carrying specific characteristics, optionally present in a sample. The invention allows the clear and unambiguous detection of at least three populations of biological elements by the use of only two detection means, which means that at least two populations of biological elements are detected by one and the same detection means. The invention can be carried out if three different probes are used, each recognizing and becoming fixed to one of the populations of biological elements to be detected, each of the probes being itself rendered detectable by a different marker, two of said markers having two emission spectra having at least one common part (overlapping emission spectra) and the third having an emission spectrum having essentially no part in common with the other two (non-overlapping spectrum).Type: ApplicationFiled: December 5, 2006Publication date: December 17, 2009Inventors: Francis Lacombe, Francis Belloc, Sylvie Veriac, Didier Lefevre
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Publication number: 20090059207Abstract: The invention relates to a device and to a method for measuring photoluminescence in a fluid present in a measurement vessel. According to the invention, the fluid in the measurement vessel simultaneously receives at least two excitation beams coming from two optical systems. The optical systems are positioned so that their axes form between them a non-zero obtuse angle other than 180° around the measurement vessel. A measurement of light emission is deduced according to the invention from coupling data obtained from emission beams picked up simultaneously by the pickup elements. The optical systems are also positioned in such a manner that there exists at least one partial overlap beam between the excitation beam from the source of a first optical system and the emission beam picked up by the pickup element of a second optical system.Type: ApplicationFiled: March 2, 2007Publication date: March 5, 2009Inventors: Philippe Nerin, Benoit Merchez, Jean-Philippe Gineys, Didier Lefevre
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Publication number: 20080283754Abstract: The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.Type: ApplicationFiled: April 14, 2006Publication date: November 20, 2008Applicant: HORIBA ABX SASInventors: Philippe Nerin, Didier Lefevre
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Publication number: 20080124745Abstract: The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.Type: ApplicationFiled: July 6, 2005Publication date: May 29, 2008Applicant: HORIBA ABX SASInventor: Didier Lefevre
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Publication number: 20070111276Abstract: The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.Type: ApplicationFiled: December 29, 2006Publication date: May 17, 2007Applicant: ABXInventors: Didier Lefevre, Sylvie Veriac, Henri Champseix
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Publication number: 20040197552Abstract: The present invention relates to a mineral fibre provided with an essentially mineral microporous or mesoporous coating and to a product comprising such fibres and optionally an organic constituent such as a binder, having a specific surface area at least equal to 10 m2/g, preferably at least equal to 30 m2/g.Type: ApplicationFiled: May 17, 2004Publication date: October 7, 2004Inventors: Bertrand Maquin, Catherine Jacquiod, Didier Lefevre, Arnaud Marchal, Olivier Larlus, Anne-Catherine Faust, Joel Patarin
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Publication number: 20020182623Abstract: The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.Type: ApplicationFiled: February 25, 2002Publication date: December 5, 2002Inventors: Didier Lefevre, Sylvie Veriac, Henri Champseix
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Patent number: 6319470Abstract: A device for automatic preparation of blood smears on plates comprises a storage unit for stacked new plates, an extractor unit to extract a plate from the stack, a depositing unit. To deposit a drop of blood on a plate, a spreading unit, to spread a drop of blood and produce a blood smear, a marking unit, comprising a marking head which can place means of identification on a reserved area of the plate, a drying unit, which can dry the blood smear, a coloring unit), which can color the blood smear, an output unit to unload the plate, and transfer means, to displace the plate between the aforementioned units, according to a pre-determined path.Type: GrantFiled: August 12, 1999Date of Patent: November 20, 2001Assignee: ABXInventors: Didier Lefevre, Ghislain Morel, Henri Champseix, Serge Champseix, Roger Le Comte
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Patent number: 5730941Abstract: It includes a measuring space (8) comprising a constrained passage (15) for the fluid, a monochromatic light source (31) delivering a beam (F1) to optical elements (6, 32, 34) including a first diffractive optical element (32) having a diffractive network (38) predetermined as a function, on the one hand, of the characteristics of the source and, on the other hand, of chosen geometrical and spectral characteristics for the illumination of the constrained passage, and suitable for inducing fluorescence of at least part of the fluid, and first (19-20), second (34-36) and third (40) optoelectronic elements intended to collect, respectively, a transmitted beam, fluorescence and a diffracted beam, and suitable for delivering to an analysis unit (24) signals relating to the contents of the beams.Type: GrantFiled: May 24, 1996Date of Patent: March 24, 1998Assignee: A B XInventors: Didier Lefevre, Bernard Kress
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Patent number: 5665309Abstract: A device for agitating and taking samples of blood products from tubes which are closed by stoppers and grouped together in a rack. The device includes a rack support adapted for holding at least one rack, at least one rack held by the rack support which is capable of holding a plurality of tubes with each tube being held in a selected position, a sampling station from which a sample of blood product can be withdrawn from a tube, a gripping device which is capable of taking hold and withdrawing from the rack one of the tubes at a selected position, bringing the tube to the sampling station and back to the selected position without agitation and replacing the tube in its selected position, and a drive device for displacing the gripping device. The device of the invention further includes a device for agitating the rack support and the rack which is operable during operation of the device for bringing and a device for withdrawing.Type: GrantFiled: February 7, 1996Date of Patent: September 9, 1997Assignee: ABXInventors: Henri Champseix, Serge Champseix, Roger Le Comte, Didier LeFevre
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Patent number: 5232857Abstract: The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.Type: GrantFiled: November 19, 1990Date of Patent: August 3, 1993Assignee: ABXInventors: Didier Lefevre, Nadine Voltas, Henri Champseix
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Patent number: 5196346Abstract: The invention relates to a reagent and a method of using the same for automatically determining the population of the basophilic leukocytes in the blood. The invention relates more particularly to a new formulation for a differential lysing reagent that lyses all the blood cells except for the basophils, particularly suitable for the measuring principle based on resistivity measurement, that is to say the concentrations of the constitutents and the pH of the solution are suitable for ensuring good electrical conductivity of the medium. The reagent according to the invention will find an application in apparatus for automatically determining blood formulae by means of resistivity variation measurements.Type: GrantFiled: February 6, 1991Date of Patent: March 23, 1993Assignee: ABXInventors: Didier Lefevre, Henri Champseix
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Patent number: 5138181Abstract: An apparatus is disclosed for counting and determining at least one leucocytic sub-population, comprising a case for injecting a flow of sample to be analyzed inside a measurement tank through which a light beam passes, comprising internal and external nozzles for sleeving the sample by means of a pressurized liquid. Electrodes for the resistivity measurements of the leucocytic sub-population are component elements of the injection case. Units for collecting and processing the signals delivered by the optical sensor and the electrodes are also provided.Type: GrantFiled: October 26, 1990Date of Patent: August 11, 1992Assignee: ABX S.A.Inventors: Didier Lefevre, Henri Champseix, Serge Champseix
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Patent number: 4520666Abstract: The invention relates to method and apparatus for determining the flow rate of fluid in a well over an interval including zones where fluid is exchanged between the well and the formations through which it is bored.A logging sonde is used to measure the temperature of the fluid along said interval, and the flow rate is measured at least at the ends of the interval. Then for each depth, a flow rate value is determined from the measured temperatures and flow rates, and the set of values obtained in this way are recorded.Type: GrantFiled: December 12, 1983Date of Patent: June 4, 1985Assignee: Schlumberger Technology Corp.Inventors: Andrea Coblentz, Didier Lefevre, Pascal Colombani, Denis Meunier