Abstract: Embodiments of the present disclosure relate to methods of screening and optionally designing compounds as inhibitors of Mfd (Mutation Frequency Decline), such compounds being useful for the treatment of bacterial infections. Embodiments of the methods include culturing pathogenic bacteria expressing functional Mfd with various concentrations of 1 mM or less, incubating the cultures with and without nitric oxide stress conditions, evaluating bacterial survival in each of the cultures, and calculating the concentration of the molecule required to decrease by 50% the survival rate of the bacteria (IC50) by comparing the cfu of the untreated cultures and cultures following nitric oxide stress.

Abstract: Embodiments of the present disclosure relate to methods of screening and optionally designing compounds as inhibitors of Mfd (Mutation Frequency Decline), such compounds being useful for the treatment of bacterial infections. Embodiments of the methods include culturing pathogenic bacteria expressing functional Mfd with various concentrations of 1 mM or less, incubating the cultures with and without nitric oxide stress conditions, evaluating bacterial survival in each of the cultures, and calculating the concentration of the molecule required to decrease by 50% the survival rate of the bacteria (IC50) by comparing the cfu of the untreated cultures and cultures following nitric oxide stress.

Abstract: The present disclosure relates to the use of a cpcR regulator gene, which directs the expression of promoters of genes encoding Cry proteins, for reducing the sporulation of a strain of Bacillus thuringiensis, new recombinant strains of Bacillus thuringiensis and uses thereof as biopesticide.

Abstract: The present invention provides nucleotide sequences from Bacillus bacteria, which control the expression of other DNA sequences in a cell host.

Abstract: This invention relates to vectors, bacterial strains, and methods for the cloning and expression of a polypeptide having larvicidal activity. In particular, the invention relates to vectors, bacterial strains and methods for the cloning and expression of the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, preferably against S. littoralis.

Abstract: This invention provides a method for obtaining a nucleotide sequence that codes for the active part of a polypeptide specifically active for larvae of S. littoralis. The method comprises hybridizing at least one nucleotide probe to DNA from a strain of B. thuringiensis active against S. littoralis, wherein at least one nucleotide probe is derived from the 5? part of a restriction fragment of a gene for ? endotoxin of B. thuringiensis strain aizawa 7-29, isolating the DNA from the strain of B. thuringiensis active against S. littoralis that hybridized to the probe, cloning the isolated DNA into a vector, and purifying the vector to thereby obtain the nucleotide sequence that codes for the active part of a polypeptide specifically active for larvae of S. littoralis, wherein the specifically active polypeptide has (1) a specificity index of less than 1.0 and (2) a higher specific toxic activity towards S. littoralis than the native crystal proteins of the strains aizawai 7-29 or berliner 1715.

Abstract: This invention relates to a method for the cloning a polypeptide having larvicidal activity. In particular, the invention relates to vectors, bacterial strains and methods for the cloning and expression of the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, preferably against S.littoralis.

Abstract: The present invention provides nucleotide sequences from Bacillus bacteria, which control the expression of other DNA sequences in a cell host.

Abstract: The present invention relates particularly to a strain of Bacillus thuringiensis characterized in that it expresses the gene sigma E (&sgr;E) and does not sporulate at all or little sporulates or does not produce any viable spores. The invention also relates to a pesticide composition containing said strain of Bacillus thuringiensis.

Abstract: The present invention provides nucleotide sequences from Bacillus bacteria, which control the expression of other DNA sequences in a cell host.

Abstract: This invention relates to plant cells, plants, and seeds expressing a polypeptide having larvicidal activity. In particular, the invention relates to plant cells, plants, and seeds expressing the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, and preferably against S.littoralis.

Abstract: A recombinant vector capable of replicating in Gram positive bacteria and containing: a) a nucleotide concatenation I of Bacillus thuringiensis with a size of about 2.6 kb between sites BalI-HpaI, or any fragment included in said concatenation as long as its allows replication of the recombinant vector when under the control of functional promoter in Gram positive bacteria, or any sequence which hybridizes with the complementary sequence of concatenation I or the above mentioned fragment under highly stringent conditions, and b) at least on DNA sequence of interest inserted in phase with the above mentioned concatenation.

Abstract: The invention relates to sequences of nucleotides of bacteria, particularly Gram positive bacteria such as bacteria of the Bacillus type and more particularly sequences of nucleotides of the gene CryIIIA for the control of the expression of DNA sequences in a cellular host. The invention relates particularly to an expression system comprising a DNA sequence susceptible of being involved in the control of the expression of a coding sequence of nucleotides. Said DNA sequence comprises a promoter, as well as a sequence of nucleotides called "downstream region", situated between the promoter and the coding sequence of the gene to be expressed, and susceptible of acting at the post-transcriptional level during the expression of the gene. Preferably, the downstream region comprises a nucleotide sequence S2 comprising an essentially complementary region at the extremity 3' of the RNA 16S of the ribosomes of Bacillus type bacteria.

Abstract: A nucleotide sequence coding for at least one part of the terminal N region of a polypeptide which exercises a specific toxic effect on Lepidoptera of the Noctuidae family, preferably on S.littoralis, is characterized by its capacity for hybridization with a gene capable of expressing a polypeptide exercising a toxic effect on S.littoralis larvae.

Abstract: The present invention relates particularly to a strain of Bacillus thuringiensis characterized in that it expresses the gene sigma E (.sigma.E) and does not sporulate at all or little sporulates or does not produce any viable spores. The invention also relates to a pesticide composition containing said strain of Bacillus thuringiensis.

Abstract: A recombinant vector capable of replicating in Gram positive bacteria and containing: a) a nucleotide concatenation I of Bacillus thuringiensis with a size of about 2.6 kb between sites BalI-HpaI, or any fragment included in said concatenation as long as its allows replication of the recombinant vector when under the control of functional promoter in Gram positive bacteria, or any sequence which hybridizes with the complementary sequence of concatenation I or the above mentioned fragment under highly stringent conditions, and b) at least on DNA sequence of interest inserted in phase with the above mentioned concatenation.

Abstract: This invention relates to plant cells, plants, and seeds expressing a polypeptide having larvicidal activity. In particular, the invention relates to plant cells, plants, and seeds expressing the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, and preferably against S.littoralis.

Abstract: A recombinant vector capable of replicating in Gram positive bacteria and containing: a) a nucleotide concatenation I of Bacillus thuringiensis with a size of about 2.6 kb between sites BalI-HpaI, or any fragment included in said concatenation as long as its allows replication of the recombinant vector when under the control of functional promoter in Gram positive bacteria, or any sequence which hybridizes with the complementary sequence of concatenation I or the above mentioned fragment under highly stringent conditions, and b) at least on DNA sequence of interest inserted in phase with the above mentioned concatenation.